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CYP7A1 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500207

CYP7A1 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

CYP7A1 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human CYP7A1 aa 50-250.

Species Reactivity

Human

Validated Applications

WB, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 58 kDa

Positive Control

LO2 cell lysate, Huh7 cell lysate, Hepa1-6 cell lysate, rat liver tissue lysate, SiHa, HeLa.

Conjugation

unconjugated

RRID

AB_3071304

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • IF-Tissue

  • 1:50-1:200

Target

Function

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body. Polymorphisms in the promoter of this gene are associated with defects in bile acid synthesis.

Background References

1. Chambers KF. et. al. Polyphenol Effects on Cholesterol Metabolism via Bile Acid Biosynthesis, CYP7A1: A Review. Nutrients. 2019 Oct

2. Zhang SH. et. al. Effects of electroacupuncture on CYP7A1 expression in liver of rabbits with atherosclerosis. Zhongguo Zhen Jiu. 2019 Jan

Subcellular Location

Endoplasmic reticulum membrane, Microsome membrane.

UNIPROT

SwissProt: P22680 Human

Synonyms

Cholesterol 7 alpha hydroxylase antibody

Cholesterol 7 alpha monooxygenase antibody

Cholesterol 7-alpha-hydroxylase antibody

Cholesterol 7-alpha-monooxygenase antibody

CP7A antibody

CP7A1_HUMAN antibody

CYP 7 antibody

CYP7 antibody

CYP7A1 antibody

CYPVII antibody

Expand

Images

  • 25 ug total protein per lane of various lysates (see on figure) probed with CYP7A1 polyclonal antibody, unconjugated (<a href="/products/HA500207" style="font-weight: bold;text-decoration: underline;">HA500207</a>) at 1:1000 dilution and 4℃ overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

    25 ug total protein per lane of various lysates (see on figure) probed with CYP7A1 polyclonal antibody, unconjugated (HA500207) at 1:1000 dilution and 4℃ overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

  • Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP7A1 Polyclonal Antibody, Unconjugated (<a href="/products/HA500207" style="font-weight: bold;text-decoration: underline;">HA500207</a>) at 1:200 overnight at 4℃, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP7A1 Polyclonal Antibody, Unconjugated (HA500207) at 1:200 overnight at 4℃, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP7A1 Polyclonal Antibody, Unconjugated (<a href="/products/HA500207" style="font-weight: bold;text-decoration: underline;">HA500207</a>) at 1:200 overnight at 4℃, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP7A1 Polyclonal Antibody, Unconjugated (HA500207) at 1:200 overnight at 4℃, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"