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PFKFB2 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500200

PFKFB2 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

PFKFB2 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human PFKFB2 aa 455-505.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P

Target Molecular Weight

58 kDa

Positive Control

MCF7 cell lysate, Hep G2 cell lysate human thyroid tissue, human uterus tissue, mouse heart tissue.

Conjugation

unconjugated

RRID

AB_3071297

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:100-1:500

Target

Function

The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene.

Background References

1. Camargo Barros-Filho M. et. al. PFKFB2 Promoter Hypomethylation as Recurrence Predictive Marker in Well-Differentiated Thyroid Carcinomas. Int J Mol Sci. 2019 Mar

2. Sreedhar A. et. al. UCP2 overexpression enhanced glycolysis via activation of PFKFB2 during skin cell transformation. Oncotarget. 2017 Sep

Subcellular Location

Cytosol, Nucleoplasm.

UNIPROT

SwissProt: O60825 Human

SwissProt: P70265 Mouse

Synonyms

6 phosphofructo 2 kinase/fructose 2 6 biphosphatase 2 antibody

6-bisphosphatase antibody

6-P2ase 2 antibody

6-P2ASE heart-type isozyme antibody

6PF 2 K/Fru 2 6 P2ASE heart type isozyme antibody

6PF-2-K/Fru-2 antibody

6PF-2-K/Fru-26-P2ase 2 antibody

6PF-2-K/Fru-26-P2ase heart-type isozyme antibody

F262_HUMAN antibody

Fructose 2 6 bisphosphatase cardiac isozyme antibody

Expand

Images

  • Western blot analysis of PFKFB2 on different lysates with Rabbit anti-PFKFB2 antibody (<a href="/products/HA500200" style="font-weight: bold;text-decoration: underline;">HA500200</a>) at 1/1,000 dilution.<br /><br />Lane 1: MCF7 (Human breast cancer cell) cell lysate<br />Lane 2: Hep G2 (Human hepatocellular carcinoma cell) cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 300 seconds; ECL: K1802<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA500200" style="font-weight: bold;text-decoration: underline;">HA500200</a>, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 58.5 kDa<br />Observed band size: 55 kDa

    Western blot analysis of PFKFB2 on different lysates with Rabbit anti-PFKFB2 antibody (HA500200) at 1/1,000 dilution.

    Lane 1: MCF7 (Human breast cancer cell) cell lysate
    Lane 2: Hep G2 (Human hepatocellular carcinoma cell) cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 300 seconds; ECL: K1802

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA500200, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 58.5 kDa
    Observed band size: 55 kDa

  • Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-PFKFB2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500200" style="font-weight: bold;text-decoration: underline;">HA500200</a>, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-PFKFB2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500200, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-PFKFB2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500200" style="font-weight: bold;text-decoration: underline;">HA500200</a>, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-PFKFB2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500200, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-PFKFB2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500200" style="font-weight: bold;text-decoration: underline;">HA500200</a>, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-PFKFB2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500200, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"