2B4 Rabbit Polyclonal Antibody
Catalog# HA500180
2B4 Rabbit Polyclonal Antibody
-
IHC-P
-
Human
-
unconjugated
Overview
Product Name
2B4 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human 2B4 aa 30-60.
Species Reactivity
Human
Validated Applications
IHC-P
Molecular Weight
42 kDa
Positive Control
Human spleen tissue, human tonsil tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
IHC-P
-
1:200-1:1,000
Target
Function
This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Background References
1. Malaer JD. et. al. 2B4 (CD244, SLAMF4) and CS1 (CD319, SLAMF7) in systemic lupus erythematosus and cancer. Clin Immunol. 2019 Jul
2. Agresta L. et. al. The Emerging Role of CD244 Signaling in Immune Cells of the Tumor Microenvironment. Front Immunol. 2018 Nov
Subcellular Location
Cell membrane, Membrane.
UNIPROT
Synonyms
2B4 antibody
C9.1 antibody
CD244 antibody
CD244 antigen antibody
CD244 molecule antibody
CD244 molecule natural killer cell receptor 2B4 antibody
CD244 natural killer cell receptor 2B4 antibody
CD244_HUMAN antibody
F730046O15Rik antibody
h2B4 antibody
ExpandImages
-
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-2B4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500180, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-2B4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500180, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
