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EVI1 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500173

EVI1 Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

EVI1 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human EVI1 aa 1-200.

Species Reactivity

Human

Validated Applications

WB

Target Molecular Weight

Predicted band size: 138 kDa

Positive Control

293 cell lysates, A431 cell lysates.

Conjugation

unconjugated

RRID

AB_3071270

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

Functions as a transcriptional regulator binding to DNA sequences in the promoter region of target genes and regulating positively or negatively their expression. Oncogene which plays a role in development, cell proliferation and differentiation. May also play a role in apoptosis through regulation of the JNK and TGF-beta signaling. Involved in hematopoiesis.

Background References

1. Kurokawa M. et. al. The oncoprotein Evi-1 represses TGF-beta signalling by inhibiting Smad3. Nature 394:92-96(1998).

Subcellular Location

Nucleus, Nucleus speckle, Cytoplasm.

UNIPROT

SwissProt: Q03112 Human

Synonyms

AML1 EVI 1 antibody

AML1 EVI 1 fusion protein antibody

AML1 EVI1 antibody

Ecotropic viral integration site 1 antibody

Ecotropic virus integration 1 site protein antibody

Ecotropic virus integration site 1 protein homolog antibody

EVI 1 antibody

EVI1 antibody

MDS1 and EVI1 complex locus antibody

MDS1 and EVI1 complex locus protein EVI1 antibody

Expand

Images

  • Western blot analysis of EVI1 on different lysates with Rabbit anti-EVI1 antibody (<a href="/products/HA500173" style="font-weight: bold;text-decoration: underline;">HA500173</a>) at 1/2,000 dilution.<br /><br />Lane 1: SK-OV-3(Human ovarian cancer cell) cell lysate<br /><br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 180 seconds; ECL: K1801<br /><br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA500173" style="font-weight: bold;text-decoration: underline;">HA500173</a>, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 138 kDa<br />Observed band size: 138 kDa

    Western blot analysis of EVI1 on different lysates with Rabbit anti-EVI1 antibody (HA500173) at 1/2,000 dilution.

    Lane 1: SK-OV-3(Human ovarian cancer cell) cell lysate


    Lysates/proteins at 20 µg/Lane.
    Exposure time: 180 seconds; ECL: K1801


    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA500173, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 138 kDa
    Observed band size: 138 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"