mGluR6 Rabbit Polyclonal Antibody
Catalog# HA500158
mGluR6 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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FC
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Human
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unconjugated
Overview
Product Name
mGluR6 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human mGluR6 aa 820-877.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, FC
Target Molecular Weight
Predicted band size: 95 kDa
Positive Control
HepG2 cell lysates, SHSY5Y.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IF-Cell
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1:50-1:200
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FC
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1:50-1:100
Target
Function
mGluR6 is specifically expressed in the retina, in a subtype of bipolar cells that depolarize in response to light, known as ON bipolar cells. These cells form synapses with photoreceptor cells, and detect the neurotransmitter glutamate via a GPCR signal transduction cascade. The glutamate receptor mGluR6 is located post-synaptically at the tips of the bipolar cell dendrites, and is responsible for initiating a signaling cascade that ultimately controls gating of the TRPM1 channel. In human patients, mutations in the GRM6 gene are associated with congenital stationary night blindness.
Background References
1. Devi S. et. al. Metabotropic glutamate receptor 6 signaling enhances TRPM1 calcium channel function and increases melanin content in human melanocytes. Pigment Cell Melanoma Res. 26:348-356(2013).
Subcellular Location
Cell membrane, Cell projection, Endoplasmic reticulum, Golgi apparatus, Membrane.
UNIPROT
Synonyms
CSNB1B antibody
DKFZp686H1993 antibody
GluR6 antibody
Glutamate receptor metabotropic 6 antibody
GPRC1F antibody
Grm6 antibody
GRM6_HUMAN antibody
Metabotropic glutamate receptor 6 antibody
mGlu6 antibody
mGluR6 antibody
Images
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Western blot analysis of mGluR6 on HepG2 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500158, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
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Flow cytometric analysis of mGluR6 was done on SHSY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500158, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"