MXD4 / MAD4 Rabbit Polyclonal Antibody
Catalog# HA500157
MXD4 / MAD4 Rabbit Polyclonal Antibody
-
WB
-
Mouse
-
Human
-
unconjugated
Overview
Product Name
MXD4 / MAD4 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human MAD4 aa 1-180.
Species Reactivity
Mouse, Human
Validated Applications
WB
Molecular Weight
Predicted band size: 24 kDa
Positive Control
Mouse brain tissue lysate, mouse lung tissue lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:1,000-1:2,000
Target
Function
This gene is a member of the MAD gene family . The MAD genes encode basic helix-loop-helix-leucine zipper proteins that heterodimerize with MAX protein, forming a transcriptional repression complex. The MAD proteins compete for MAX binding with MYC, which heterodimerizes with MAX forming a transcriptional activation complex. Studies in rodents suggest that the MAD genes are tumor suppressors and contribute to the regulation of cell growth in differentiating tissues.
Background References
1. Tamura R. et. al. Novel MXD4-NUTM1 fusion transcript identified in primary ovarian undifferentiated small round cell sarcoma. Genes Chromosomes Cancer. 2018 Nov
2. Boros K. et. al. The transcription factor Mxd4 controls the proliferation of the first blood precursors at the onset of hematopoietic development in vitro. Exp Hematol. 2011 Nov
Subcellular Location
Nucleus.
Synonyms
bHLHc12 antibody
Class C basic helix loop helix protein 12 antibody
Class C basic helix-loop-helix protein 12 antibody
MAD 4 antibody
MAD4 antibody
Mad4 homolog antibody
MAD4_HUMAN antibody
Max associated protein 4 antibody
MAX dimerization protein 4 antibody
Max dimerizer 4 antibody
ExpandImages
-
Western blot analysis of MXD4 / MAD4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500157, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse lung tissue lysate
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"