GNB3 Rabbit Polyclonal Antibody
Catalog# HA500153
GNB3 Rabbit Polyclonal Antibody
-
WB
-
IHC-P
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
GNB3 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human GNB3 aa 10-60.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
37 kDa
Positive Control
HepG2 cell lysate, Hela cell lysate, mouse brain tissue lysate, human brain tissue lysate, rat brain tissue lysate, rat kidney tissue, mouse brain tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:1,000-1:2,000
-
IHC-P
-
1:100-1:500
Target
Function
Heterotrimeric guanine nucleotide-binding proteins ( G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. A single-nucleotide polymorphism (C825T) in this gene is associated with essential hypertension and obesity. This polymorphism is also associated with the occurrence of the splice variant GNB3-s, which appears to have increased activity. GNB3-s is an example of alternative splicing caused by a nucleotide change outside of the splice donor and acceptor sites. Additional splice variants may exist for this gene, but they have not been fully described.
Background References
1. Hill C. et. al. The cationic region of Rhes mediates its interactions with specific Gbeta subunits. Cell. Physiol. Biochem. 23:1-8(2009).
Subcellular Location
Cytosol.
Synonyms
G protein beta 3 subunit antibody
GBB3_HUMAN antibody
GNB3 antibody
GNB3 guanine nucleotide binding protein (G protein), beta polypeptide 3 antibody
GTP binding regulatory protein beta 3 chain antibody
Guanine nucleotide binding protein (G protein) beta polypeptide 3 antibody
Guanine nucleotide binding protein G antibody
Guanine nucleotide binding protein G(I)/G(S)/G(T) beta subunit 3 antibody
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3 antibody
Hypertension associated protein antibody
ExpandImages
-
Western blot analysis of GNB3 on dHep G2 (Human hepatocellular carcinoma cell) cell lysate with Rabbit anti-GNB3 antibody (HA500153) at 1/5,000 dilution.
Lysates/proteins at 10 µg/Lane.
Exposure time: 2 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500153, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/20,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 37 kDa
Observed band size: 37 kDa -
Western blot analysis of GNB3 on different lysates with Rabbit anti-GNB3 antibody (HA500153) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 2minutes6seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500153, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 37 kDa
Observed band size: 37 kDa -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-GNB3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500153, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GNB3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500153, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
