ASIC4 Rabbit Polyclonal Antibody
Catalog# HA500151
ASIC4 Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
ASIC4 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human ASIC4 aa 250-450.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
70 kDa
Positive Control
HepG2 cell lysate, K562 cell lysate, rat brain tissue, mouse pituitary tissue, SH-SY5Y.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1,000-1:5,000
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IHC-P
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1:100-1:500
Target
Function
This gene belongs to the superfamily of acid-sensing ion channels, which are proton-gated, amiloride-sensitive sodium channels. These channels have been implicated in synaptic transmission, pain perception as well as mechanoperception. This gene is predominantly expressed in the pituitary gland, and was considered a candidate for paroxysmal dystonic choreoathetosis (PDC), a movement disorder, however, no correlation was found between mutations in this gene and PDC.
Background References
1. Wang LM. et. al. Walnut oil improves spatial memory in rats and increases the expression of acid-sensing ion channel genes Asic2a and Asic4. Food Sci Nutr. 2018 Nov
2. Hoshikawa M. et. al. Distribution of ASIC4 transcripts in the adult wild-type mouse brain. Neurosci Lett. 2017 Jun
Subcellular Location
Membrane.
Synonyms
Acid-sensing ion channel 4
ASIC4
Amiloride-sensitive cation channel 4
Amiloride-sensitive cation channel 4, pituitary
ACCN4
Images
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Western blot analysis of ASIC4 on different lysates with Rabbit anti-ASIC4 antibody (HA500151) at 1/5,000 dilution.
Lane 1: Hep G2 (Human hepatocellular carcinoma cell) cell lysate
Lane 2: K-562 (Human chronic myelogenous leukemia cell) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 110 seconds ; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500151, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size:59 kDa
Observed band size:70 kDa -
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ASIC4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500151, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue using anti-ASIC4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500151, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"