ERAB Rabbit Polyclonal Antibody
Catalog# HA500150
ERAB Rabbit Polyclonal Antibody
-
WB
-
Human
-
unconjugated
Overview
Product Name
ERAB Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human ERAB aa 1-200.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 27 kDa
Positive Control
A549 cell lysate, MCF-7 cell lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:500-1:1,000
Target
Function
In addition to mitochondrial dehydrogenase activity, moonlights as a component of mitochondrial ribonuclease P, a complex that cleaves tRNA molecules in their 5'-ends . Together with TRMT10C/MRPP1, forms a subcomplex of the mitochondrial ribonuclease P, named MRPP1-MRPP2 subcomplex, which displays functions that are independent of the ribonuclease P activity . The MRPP1-MRPP2 subcomplex catalyzes the formation of N1-methylguanine and N1-methyladenine at position 9 (m1G9 and m1A9, respectively) in tRNAs; HSD17B10/MRPP2 acting as a non-catalytic subunit . The MRPP1-MRPP2 subcomplex also acts as a tRNA maturation platform: following 5'-end cleavage by the mitochondrial ribonuclease P complex, the MRPP1-MRPP2 subcomplex enhances the efficiency of 3'-processing catalyzed by ELAC2, retains the tRNA product after ELAC2 processing and presents the nascent tRNA to the mitochondrial CCA tRNA nucleotidyltransferase TRNT1 enzyme . Associates with mitochondrial DNA complexes at the nucleoids to initiate RNA processing and ribosome assembly.
Background References
1. Holzmann J. et. al. RNase P without RNA: identification and functional reconstitution of the human mitochondrial tRNA processing enzyme. Cell 135:462-474(2008).
Subcellular Location
Mitochondrion, Mitochondrion matrix, mitochondrion nucleoid.
Synonyms
17 beta hydroxysteroid dehydrogenase 10 antibody
17 beta hydroxysteroid dehydrogenase type 10 antibody
17-beta-HSD 10 antibody
17-beta-hydroxysteroid dehydrogenase 10 antibody
17b HSD10 antibody
3 hydroxy 2 methylbutyryl CoA dehydrogenase antibody
3 hydroxyacyl CoA dehydrogenase type 2 antibody
3 hydroxyacyl CoA dehydrogenase type II antibody
3-hydroxy-2-methylbutyryl-CoA dehydrogenase antibody
3-hydroxyacyl-CoA dehydrogenase type II antibody
ExpandImages
-
Western blot analysis of ERAB on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500150, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A549 cell lysate
Lane 2: MCF-7 cell lysate
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
