PIP5K2 gamma Rabbit Polyclonal Antibody
Catalog# HA500139
PIP5K2 gamma Rabbit Polyclonal Antibody
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
PIP5K2 gamma Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human PIP5K2 gamma aa 100-300.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
47 kDa
Positive Control
MCF-7 cell lysate, A549 cell lysate, rat cerebellum tissue lysate, mouse brain tissue lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1,000-1:2,000
Target
Function
PIP4K2C (Phosphatidylinositol-5-Phosphate 4-Kinase Type 2 Gamma) is a Protein Coding gene. Phosphatidylinositol 5-phosphate 4-kinase with low enzymatic activity. May be a GTP sensor, has higher GTP-dependent kinase activity than ATP-dependent kinase activity. Diseases associated with PIP4K2C include Parkinson Disease 20, Early-Onset and Epileptic Encephalopathy, Early Infantile, 53. Among its related pathways are Regulation of TP53 Activity through Acetylation and RET signaling. Gene Ontology (GO) annotations related to this gene include identical protein binding and 1-phosphatidylinositol-5-phosphate 4-kinase activity. An important paralog of this gene is PIP4K2B.
Background References
1. Sumita K. et. al. The Lipid Kinase PI5P4Kbeta Is an Intracellular GTP Sensor for Metabolism and Tumorigenesis. Mol. Cell 61:187-198(2016).
Subcellular Location
Cytoplasm, Endoplasmic reticulum.
Synonyms
Phosphatidylinositol 4 phosphate 5 kinase antibody
Phosphatidylinositol 4 phosphate 5 kinase type II gamma antibody
Phosphatidylinositol-5-phosphate 4-kinase type II gamma antibody
Phosphatidylinositol-5-phosphate 4-kinase type-2 gamma antibody
PI(5)P 4-kinase type II gamma antibody
PI42C_HUMAN antibody
Pip4k2c antibody
PIP4KII-gamma antibody
PIP5K2C antibody
Images
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Western blot analysis of PIP5K2 gamma on different lysates with Rabbit anti-PIP5K2 gamma antibody (HA500139) at 1/1,000 dilution.
Lane 1: MCF7 (Human breast cancer cell) cell lysate
Exposure time: 20 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500139, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 47.3 kDa
Observed band size: 47 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"