GXYLT1 Rabbit Polyclonal Antibody
Catalog# HA500113
GXYLT1 Rabbit Polyclonal Antibody
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WB
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Human
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unconjugated
Overview
Product Name
GXYLT1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human GXYLT1 aa 400-440.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 51 kDa
Positive Control
U-937 cell lysate, HL-60 cell lysate, HUVEC cell lysate, LNCaP cell lysate, HepG2 cell lysate, HeLa cell lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:5,000
Target
Function
Glycosyltransferase which elongates the O-linked glucose attached to EGF-like repeats in the extracellular domain of Notch proteins by catalyzing the addition of xylose. GXYLT1 is a xylosyltransferase (EC 2.4.2.-) that adds the first xylose to O-glucose-modified residues in the epidermal growth factor (EGF; MIM 131530) repeats of proteins such as NOTCH1. GXYLT1 (Glucoside Xylosyltransferase 1) is a Protein Coding gene. Diseases associated with GXYLT1 include Dowling-Degos Disease and Adams-Oliver Syndrome. Among its related pathways are Mucin type O-glycan biosynthesis. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring glycosyl groups and UDP-xylosyltransferase activity. An important paralog of this gene is GXYLT2.
Background References
1. Sethi M.K. et. al. Identification of glycosyltransferase 8 family members as xylosyltransferases acting on O-glucosylated notch epidermal growth factor repeats.J. Biol. Chem. 285:1582-1586(2010).
Subcellular Location
Membrane.
UNIPROT
Synonyms
Glycosyltransferase 8 domain-containing protein 3 antibody
Glucoside xylosyltransferase 1 antibody
GXYLT1 antibody
GLT8D3 antibody
Images
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Western blot analysis of GXYLT1 on different lysates with Rabbit anti-GXYLT1 antibody (HA500113) at 1/5,000 dilution.
Lane 1: U-937 cell lysate
Lane 2: HL-60 cell lysate
Lane 3: HUVEC cell lysate
Lane 4: LNCaP cell lysate
Lane 5: HepG2 cell lysate
Lane 6: HeLa cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500113) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"