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AMPS Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500092

AMPS Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

AMPS Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within N-terminal human AMPS.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell

Target Molecular Weight

Predicted band size: 55 kDa

Positive Control

Jurkat cell lysate, 293T cell lysate, HeLa cell lysate, Mouse skeletal muscle tissue lysate, Rat skeletal muscle tissue lysate, HeLa.

Conjugation

unconjugated

RRID

AB_3071191

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Cell

  • 1:200

Target

Function

Adenylosuccinate lyase (ASL) is an enzyme that catalyzes two reactions in the de novo purine biosynthetic pathway. In both reactions it uses an E1cb elimination reaction mechanism to cleave fumarate off of the substrate. In the first reaction, ASL converts 5-aminoimidazole- (N-succinylocarboxamide) ribotide (SAICAR) to 5-aminoimidazole-4-carboxamide ribotide (AICAR) and fumarate. AICAR proceeds through three more reactions before it becomes adenylosuccinate (also called succinyladenosine monophosphate or SAMP), which ASL then splits into adenosine monophosphate (AMP) and fumarate. ASL is important to cells not only because of its involvement in creating purines needed for cellular replication, but also because it helps regulate metabolic processes by controlling the levels of AMP and fumarate in the cell.

Background References

1. Kmoch S, et. al. Human adenylosuccinate lyase (ADSL), cloning and characterization of full-length cDNA and its isoform, gene structure and molecular basis for ADSL deficiency in six patients.Hum. Mol. Genet. 9:1501-1513(2000)

Subcellular Location

Cytosol.

UNIPROT

SwissProt: P30566 Human

SwissProt: P54822 Mouse

Entrez Gene: 315150 Rat

Synonyms

Adenylosuccinase antibody

Adenylosuccinate lyase antibody

ADSL antibody

AMPS antibody

ASase antibody

ASL antibody

OTTHUMP00000199172 antibody

OTTHUMP00000199173 antibody

PUR8_HUMAN antibody

Images

  • Western blot analysis of AMPS on different lysates with Rabbit anti-AMPS antibody (<a href="/products/HA500092" style="font-weight: bold;text-decoration: underline;">HA500092</a>) at 1/2,000 dilution.<br /><br />Lane 1: Jurkat cell lysate (20 µg/Lane)<br />Lane 2: 293T cell lysate (20 µg/Lane)<br />Lane 3: HeLa cell lysate (20 µg/Lane)<br />Lane 4: Mouse skeletal muscle tissue lysate (40 µg/Lane)<br />Lane 5: Rat skeletal muscle tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 55 kDa<br />Observed band size: 54 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA500092" style="font-weight: bold;text-decoration: underline;">HA500092</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of AMPS on different lysates with Rabbit anti-AMPS antibody (HA500092) at 1/2,000 dilution.

    Lane 1: Jurkat cell lysate (20 µg/Lane)
    Lane 2: 293T cell lysate (20 µg/Lane)
    Lane 3: HeLa cell lysate (20 µg/Lane)
    Lane 4: Mouse skeletal muscle tissue lysate (40 µg/Lane)
    Lane 5: Rat skeletal muscle tissue lysate (40 µg/Lane)

    Predicted band size: 55 kDa
    Observed band size: 54 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500092) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of HeLa cells labeling AMPS with Rabbit anti-AMPS antibody (<a href="/products/HA500092" style="font-weight: bold;text-decoration: underline;">HA500092</a>) at 1/200 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AMPS antibody (<a href="/products/HA500092" style="font-weight: bold;text-decoration: underline;">HA500092</a>) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HeLa cells labeling AMPS with Rabbit anti-AMPS antibody (HA500092) at 1/200 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AMPS antibody (HA500092) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"