HOXA9 Rabbit Polyclonal Antibody
Catalog# HA500077
HOXA9 Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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Mouse
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unconjugated
Overview
Product Name
HOXA9 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human HOXA9 aa 20-60.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 30 kDa
Positive Control
293T (Human embryonic kidney cell) cell lysate, mouse kidney tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IHC-P
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1:100-1:500
Target
Function
HOXA9, also known HOX1G, which belongs to the Abd-B homeobox family and transiently interacts with PRMT5 in TNF-alpha stimulated endothelial cells. HOXA9 is methylated on Arg-140 by PRMT5and methylation is critical for E-selectin induction. HOXA9 as a sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. HOXA9 is required for induction of E-selectin and VCAM-1, on the endothelial cells surface at sites of inflammation. HOXA9 is 30 kDa, but methylation of HOXA9 is about 35-40 kDa.
Background References
1. Bandyopadhyay S. et. al. HOXA9 methylation by PRMT5 is essential for endothelial cell expression of leukocyte adhesion molecules. Mol. Cell. Biol. 32:1202-1213(2012).
Subcellular Location
Nucleus.
Synonyms
ABD-B antibody
Abd-B, drosophila, homolog of antibody
D6a9 antibody
Homeobox 1G antibody
Homeobox A9 antibody
Homeobox protein Hox-1.7 antibody
Homeobox protein Hox-1G antibody
Homeobox protein Hox-A9 antibody
Homeodomain protein HOXA9 antibody
Hox-1.7 antibody
ExpandImages
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Western blot analysis of HOXA9 on 293T (Human embryonic kidney cell) cell lysate with Rabbit anti-HOXA9 antibody (HA500077) at 1/500 dilution.
Lysates/proteins at 10 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500077, 1/500 in 5% NFDM/TBST, 2 hours at room temperature
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/40,000 in 5% NFDM/TBST, 1 hour at room temperature -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-HOXA9 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500077, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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