IL-2 receptor alpha Rabbit Polyclonal Antibody
Catalog# HA500076
IL-2 receptor alpha Rabbit Polyclonal Antibody
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WB
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Human
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Rat
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unconjugated
Overview
Product Name
IL-2 receptor alpha Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human IL-2 receptor alpha aa 22-70 (Extracellular domain).
Species Reactivity
Human, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 31 kDa
Positive Control
Human kidney tissue lysate, rat spleen tissue lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:1,000
Target
Function
Interleukin-2 receptor alpha chain (also called CD25) is a protein that in humans is encoded by the IL2RA gene.The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolysis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is currently unknown.
Background References
1. Goudy K. et. al. Human IL2RA null mutation mediates immunodeficiency with lymphoproliferation and autoimmunity. Clin. Immunol. 146:248-261(2013).
Subcellular Location
Membrane.
Synonyms
Interleukin 2 receptor alpha chain antibody
CD25 antibody
CD25 antigen antibody
IDDM10 antibody
IL 2 receptor alpha subunit antibody
IL-2 receptor subunit alpha antibody
IL-2-RA antibody
IL-2R subunit alpha antibody
IL2 RA antibody
IL2 Receptor alpha antibody
ExpandImages
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Western blot analysis of IL-2 receptor alpha on Rat cerebellum tissue lysate with Rabbit anti-IL-2 receptor alpha antibody (HA500076) at 1/1,000 dilution.
Lysates/proteins at 40 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500076, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature -
Western blot analysis of IL-2 receptor alpha on Rat cerebellum tissue lysate with Rabbit anti-IL-2 receptor alpha antibody (HA500076) at 1/500 dilution.
Lysates/proteins at 20 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500076, 1/500 in 5% NFDM/TBST, 2 hours at room temperature
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/40,000 in 5% NFDM/TBST, 1 hour at room temperature
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
CD25 / IL-2 Receptor alpha Recombinant Mouse Monoclonal Antibody [PSH14-46]
Application: FC,IHC-P,IF-Tissue
Reactivity: Human,Mouse
Conjugate: unconjugated
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