IL-2 receptor alpha Rabbit Polyclonal Antibody
Catalog# HA500076
IL-2 receptor alpha Rabbit Polyclonal Antibody
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WB
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Human
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Rat
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unconjugated
Overview
Product Name
IL-2 receptor alpha Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human IL-2 receptor alpha aa 22-70 (Extracellular domain).
Species Reactivity
Human, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 31 kDa
Positive Control
Human kidney tissue lysate, rat spleen tissue lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:1,000
Target
Function
Interleukin-2 receptor alpha chain (also called CD25) is a protein that in humans is encoded by the IL2RA gene.The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolysis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is currently unknown.
Background References
1. Goudy K. et. al. Human IL2RA null mutation mediates immunodeficiency with lymphoproliferation and autoimmunity. Clin. Immunol. 146:248-261(2013).
Subcellular Location
Membrane.
Synonyms
Interleukin 2 receptor alpha chain antibody
CD25 antibody
CD25 antigen antibody
IDDM10 antibody
IL 2 receptor alpha subunit antibody
IL-2 receptor subunit alpha antibody
IL-2-RA antibody
IL-2R subunit alpha antibody
IL2 RA antibody
IL2 Receptor alpha antibody
ExpandImages
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Western blot analysis of IL-2 receptor alpha on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500076, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human kidney tissue lysate
Lane 2: Rat spleen tissue lysate
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"