PDE2A Rabbit Polyclonal Antibody
Catalog# HA500055
PDE2A Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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Mouse
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unconjugated
Overview
Product Name
PDE2A Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human PDE2A aa 750-941.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 105 kDa
Positive Control
Mouse spleen tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, human brain tissue, human kidney tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000-1:50,000
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IHC-P
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1:1,000
Target
Function
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Plays an important role in growth and invasion of malignant melanoma cells (e.g. pseudomyxoma peritonei (PMP) cell line).
Background References
1. Hiramoto K. et. al. Role of phosphodiesterase 2 in growth and invasion of human malignant melanoma cells. Cell. Signal. 26:1807-1817(2014).
Subcellular Location
Cell membrane, Cytoplasm, Membrane, Mitochondrion, Mitochondrion inner membrane, Mitochondrion outer membrane.
Synonyms
5''-cyclic phosphodiesterase antibody
cGMP dependent 3'5' cyclic phosphodiesterase antibody
cGMP stimulated phosphodiesterase 1 antibody
cGMP stimulated phosphodiesterase 4 antibody
cGMP-dependent 3'' antibody
cGMP-stimulated phosphodiesterase 2 antibody
CGS PDE antibody
CGS-PDE antibody
cGSPDE antibody
Cyclic GMP stimulated phosphodiesterase antibody
ExpandImages
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Western blot analysis of PDE2A on different lysates with Rabbit anti-PDE2A antibody (HA500055) at 1/50,000 dilution.
Lane 1: mouse spleen tissue lysate
Lane 2: mouse brain tissue lysate
Lane 3: rat brain tissue lysate
Lysates/proteins at 30 µg/Lane.
Exposure time: 60 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500055, 1/50,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 105.7 kDa
Observed band size: 105 kDa -
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PDE2A antibody (HA500055) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500055) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PDE2A antibody (HA500055) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500055) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"