Nodal Rabbit Polyclonal Antibody
Catalog# HA500041
Nodal Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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Rat
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unconjugated
Overview
Product Name
Nodal Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human Nodal aa 150-347.
Species Reactivity
Human, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 40 kDa
Positive Control
Rat testis tissue cell lysate, SHSY5Y cell lysate, rat testis tissue, human colon tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IHC-P
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1:50-1:200
Target
Function
Nodal is a secretory protein that in humans is encoded by the NODAL genewhich is located on chromosome 10q22.1. It belongs to the transforming growth factor beta (TGF-β) superfamily. Like many other members of this superfamily it is involved in cell differentiation in early embryogenesis, playing a key role in signal transfer from the node, in the anterior primitive streak, to lateral plate mesoderm (LPM).Nodal signaling is important very early in development for mesoderm and endoderm formation and subsequent organization of left-right axial structures.In addition, Nodal seems to have important functions in neural patterning, stem cell maintenanceand many other developmental processes, including left/right handedness.
Background References
1. Gebbia M. et. al. X-linked situs abnormalities result from mutations in ZIC3. Nat. Genet. 17:305-308(1997).
Subcellular Location
Secreted.
UNIPROT
Synonyms
HTX5 antibody
MGC138230 antibody
Nodal antibody
Nodal Growth Differentiation Factor antibody
nodal homolog (mouse) antibody
Nodal homolog antibody
Nodal, Mouse, Homolog antibody
NODAL_HUMAN antibody
Images
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Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Nodal antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500041, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Nodal antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500041, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Western blot analysis of Nodal on different lysates with Rabbit anti-Nodal antibody (HA500041) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: SH-SY5Y cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 180 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500041, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 40 kDa
Observed band size: 40 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"