Dynamin 3 Rabbit Polyclonal Antibody
Catalog# HA500037
Dynamin 3 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Dynamin 3 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human Dynamin 3 aa 750-800.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 97 kDa
Positive Control
Mouse cerebellum tissue lysate, Rat cerebellum tissue lysate, SKOV-3.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1,000-1:5,000
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IF-Cell
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1:50-1:200
Target
Function
Dynamin-3 is a protein that in humans is encoded by the DNM3 gene. The protein encoded by this gene is a member of the dynamin family which possess mechanochemical properties involved in actin-membrane processes, predominantly in membrane budding. DNM3 is upregulated in Sézary's syndrome.Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis (By similarity).
Background References
1. Noah W Gray. et. al. Dynamin 3 is a component of the postsynapse, where it interacts with mGluR5 and Homer. Curr Biol. 2003 Mar 18;13(6):510-5.
Subcellular Location
Cytoplasm, Cytoskeleton, Microtubule.
Synonyms
testicular dynamin antibody
testicular antibody
DNM 3 antibody
DNM3 antibody
DNM3 protein antibody
Dyn3 antibody
DYN3_HUMAN antibody
Dyna III antibody
DynaIII antibody
dynamin 2 antibody
ExpandImages
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Western blot analysis of Dynamin 3 on different lysates with Rabbit anti-Dynamin 3 antibody (HA500037) at 1/5,000 dilution.
Lane 1: Mouse cerebellum tissue lysate
Lane 2: Mouse small intestine tissue lysate
Lane 3: Rat cerebellum tissue lysate
Lysates/proteins at 30 µg/Lane.
Exposure time: 59 seconds; ECL: K1801
The expression level on Mouse small intestine (low) is consistent with literature (PMID: 21689597).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500037, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 97.7 kDa
Observed band size: 98 kDa -
ICC staining of Dynamin 3 in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500037, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
Dynamin 3 Recombinant Rabbit Monoclonal Antibody [JE54-86]
Application: WB
Reactivity: Human,Mouse
Conjugate: unconjugated
Products with the same target and pathway
Dynamin 3 Recombinant Rabbit Monoclonal Antibody [JE54-86]
Application: WB
Reactivity: Human,Mouse
Conjugate: unconjugated
