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Dynamin 3 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500032

Dynamin 3 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Dynamin 3 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human Dynamin 3 aa 820-869.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

97 kDa

Positive Control

SH-SY5Y (Human neuroblastoma cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, Mouse brain tissue lysate,Rat brain tissue lysate, mouse brain tissue.

Conjugation

unconjugated

RRID

AB_3071132

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:5,000

  • IHC-P

  • 1:50-1:200

Target

Function

Dynamin-3 is a protein that in humans is encoded by the DNM3 gene. The protein encoded by this gene is a member of the dynamin family which possess mechanochemical properties involved in actin-membrane processes, predominantly in membrane budding. DNM3 is upregulated in Sézary's syndrome.Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis (By similarity).

Background References

1. Noah W Gray. et. al. Dynamin 3 is a component of the postsynapse, where it interacts with mGluR5 and Homer. Curr Biol. 2003 Mar 18;13(6):510-5.

Subcellular Location

Cytoplasm, Cytoskeleton, Microtubule.

UNIPROT

SwissProt: Q9UQ16 Human

SwissProt: Q8BZ98 Mouse

SwissProt: Q08877 Rat

Synonyms

testicular dynamin antibody

testicular antibody

DNM 3 antibody

DNM3 antibody

DNM3 protein antibody

Dyn3 antibody

DYN3_HUMAN antibody

Dyna III antibody

DynaIII antibody

dynamin 2 antibody

Expand

Images

  • Western blot analysis of Dynamin 3 on SH-SY5Y (Human neuroblastoma cell) cell lysate with Rabbit anti-Dynamin 3 antibody (<a href="/products/HA500032" style="font-weight: bold;text-decoration: underline;">HA500032</a>) at 1/5,000 dilution.<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 10 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA500032" style="font-weight: bold;text-decoration: underline;">HA500032</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 97 kDa<br />Observed band size: 97 kDa

    Western blot analysis of Dynamin 3 on SH-SY5Y (Human neuroblastoma cell) cell lysate with Rabbit anti-Dynamin 3 antibody (HA500032) at 1/5,000 dilution.

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 10 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA500032, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 97 kDa
    Observed band size: 97 kDa

  • Western blot analysis of Dynamin 3 on NIH/3T3 (Mouse fibroblast) cell lysate with Rabbit anti-Dynamin 3 antibody (<a href="/products/HA500032" style="font-weight: bold;text-decoration: underline;">HA500032</a>) at 1/5,000 dilution.<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 10 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA500032" style="font-weight: bold;text-decoration: underline;">HA500032</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 97 kDa<br />Observed band size: 97 kDa

    Western blot analysis of Dynamin 3 on NIH/3T3 (Mouse fibroblast) cell lysate with Rabbit anti-Dynamin 3 antibody (HA500032) at 1/5,000 dilution.

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 10 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA500032, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 97 kDa
    Observed band size: 97 kDa

  • Western blot analysis of Dynamin 3 on different lysates with Rabbit anti-Dynamin 3 antibody (<a href="/products/HA500032" style="font-weight: bold;text-decoration: underline;">HA500032</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Rat brian tissue lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br />Exposure time: 2 seconds; ECL: K1801<br /><br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA500032" style="font-weight: bold;text-decoration: underline;">HA500032</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 97 kDa<br />Observed band size: 97 kDa

    Western blot analysis of Dynamin 3 on different lysates with Rabbit anti-Dynamin 3 antibody (HA500032) at 1/5,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Rat brian tissue lysate

    Lysates/proteins at 30 µg/Lane.
    Exposure time: 2 seconds; ECL: K1801


    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA500032, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 97 kDa
    Observed band size: 97 kDa

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Dynamin 3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500032" style="font-weight: bold;text-decoration: underline;">HA500032</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Dynamin 3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500032, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Dynamin 3 Recombinant Rabbit Monoclonal Antibody [JE54-86]

Application: WB

Reactivity: Human,Mouse

Conjugate: unconjugated

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Dynamin 3 Recombinant Rabbit Monoclonal Antibody [JE54-86]

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Dynamin 3 Rabbit Polyclonal Antibody

Application: WB,IF-Cell

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Conjugate: unconjugated