CAPZB Rabbit Polyclonal Antibody
Catalog# HA500003
CAPZB Rabbit Polyclonal Antibody
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WB
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Human
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unconjugated
Overview
Product Name
CAPZB Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human CAPZB aa 30-80.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
31 kDa
Positive Control
A431 cell lysate, THP-1 cell lysate, Jurkat cell lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:500-1:1,000
Target
Function
F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. Plays a role in the regulation of cell morphology and cytoskeletal organization.
Background References
1. Bai S.W. et. al. Identification and characterization of a set of conserved and new regulators of cytoskeletal organisation, cell morphology and migration. BMC Biol. 9:54-54(2011).
Subcellular Location
Cytoplasm, Cytoskeleton.
UNIPROT
Synonyms
11 antibody
Cap protein, actin, beta antibody
Cap Z antibody
CAPB antibody
CAPPB antibody
Capping protein (actin filament) muscle Z line beta antibody
Capping protein beta 2 antibody
CAPZ antibody
CapZ beta antibody
Capzb antibody
ExpandImages
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Western blot analysis of CAPZB on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500003, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A431 cell lysate
Lane 2: THP-1 cell lysate
Lane 3: Jurkat cell lysate
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"