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UCHL3 Recombinant Rabbit Monoclonal Antibody [JE55-94]

Usd: 385

Specification

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Catalog# ET7111-28

UCHL3 Recombinant Rabbit Monoclonal Antibody [JE55-94]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

UCHL3 Recombinant Rabbit Monoclonal Antibody [JE55-94]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within C-terminal Human UCHL3.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 26 kDa

Positive Control

Daudi cell lysate, HeLa cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, Mouse colon tissue lysate, Rat heart tissue lysate, Rat testis tissue lysate.

Conjugation

unconjugated

Clone Number

JE55-94

RRID

AB_3071085

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

The protein encoded by this gene is a member of the deubiquitinating enzyme family. Members of this family are proteases that catalyze the removal of ubiquitin from polypeptides and are divided into five classes, depending on the mechanism of catalysis. This protein may hydrolyze the ubiquitinyl-N-epsilon amide bond of ubiquitinated proteins to regenerate ubiquitin for another catalytic cycle. Alternative splicing results in multiple transcript variants that encode different protein isoforms.

Background References

1. Nishi R. et. al. The deubiquitylating enzyme UCHL3 regulates Ku80 retention at sites of DNA damage. Sci Rep. 2018 Dec

2. Liao C. et. al. UCHL3 Regulates Topoisomerase-Induced Chromosomal Break Repair by Controlling TDP1 Proteostasis. Cell Rep. 2018 Jun

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P15374 Human

SwissProt: Q9JKB1 Mouse

SwissProt: Q91Y78 Rat

Synonyms

Ubiquitin carboxyl-terminal hydrolase isozyme L3 antibody

Ubiquitin thioesterase L3 antibody

Ubiquitin thiolesterase antibody

Ubiquitin thiolesterase L3 antibody

UCH L3 antibody

UCH-L3 antibody

UCHL3 antibody

UCHL3_HUMAN antibody

Images

  • Western blot analysis of UCHL3 on different lysates with Rabbit anti-UCHL3 antibody (<a href="/products/ET7111-28" style="font-weight: bold;text-decoration: underline;">ET7111-28</a>) at 1/1,000 dilution.<br /><br />Lane 1: Daudi cell lysate (20 µg/Lane)<br />Lane 2: HeLa cell lysate (20 µg/Lane)<br />Lane 3: Neuro-2a cell lysate (20 µg/Lane)<br />Lane 4: PC-12 cell lysate (20 µg/Lane)<br />Lane 5: Mouse colon tissue lysate (40 µg/Lane)<br />Lane 6: Rat heart tissue lysate (40 µg/Lane)<br />Lane 7: Rat testis tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 26 kDa<br />Observed band size: 26 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7111-28" style="font-weight: bold;text-decoration: underline;">ET7111-28</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of UCHL3 on different lysates with Rabbit anti-UCHL3 antibody (ET7111-28) at 1/1,000 dilution.

    Lane 1: Daudi cell lysate (20 µg/Lane)
    Lane 2: HeLa cell lysate (20 µg/Lane)
    Lane 3: Neuro-2a cell lysate (20 µg/Lane)
    Lane 4: PC-12 cell lysate (20 µg/Lane)
    Lane 5: Mouse colon tissue lysate (40 µg/Lane)
    Lane 6: Rat heart tissue lysate (40 µg/Lane)
    Lane 7: Rat testis tissue lysate (40 µg/Lane)

    Predicted band size: 26 kDa
    Observed band size: 26 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-28) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of UCHL3 on different lysates with Rabbit anti-UCHL3 antibody (<a href="/products/ET7111-28" style="font-weight: bold;text-decoration: underline;">ET7111-28</a>) at 1/5,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-UCHL3 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 26 kDa<br />Observed band size: 26 kDa<br /><br />Exposure time: 180 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7111-28" style="font-weight: bold;text-decoration: underline;">ET7111-28</a>) at 1/5,000 dilution was used in <a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a> at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of UCHL3 on different lysates with Rabbit anti-UCHL3 antibody (ET7111-28) at 1/5,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-UCHL3 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 26 kDa
    Observed band size: 26 kDa

    Exposure time: 180 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-28) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"