UCHL3 Recombinant Rabbit Monoclonal Antibody [JE55-94]
Catalog# ET7111-28
UCHL3 Recombinant Rabbit Monoclonal Antibody [JE55-94]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
UCHL3 Recombinant Rabbit Monoclonal Antibody [JE55-94]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within C-terminal Human UCHL3.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 26 kDa
Positive Control
Daudi cell lysate, HeLa cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, Mouse colon tissue lysate, Rat heart tissue lysate, Rat testis tissue lysate.
Conjugation
unconjugated
Clone Number
JE55-94
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
The protein encoded by this gene is a member of the deubiquitinating enzyme family. Members of this family are proteases that catalyze the removal of ubiquitin from polypeptides and are divided into five classes, depending on the mechanism of catalysis. This protein may hydrolyze the ubiquitinyl-N-epsilon amide bond of ubiquitinated proteins to regenerate ubiquitin for another catalytic cycle. Alternative splicing results in multiple transcript variants that encode different protein isoforms.
Background References
1. Nishi R. et. al. The deubiquitylating enzyme UCHL3 regulates Ku80 retention at sites of DNA damage. Sci Rep. 2018 Dec
2. Liao C. et. al. UCHL3 Regulates Topoisomerase-Induced Chromosomal Break Repair by Controlling TDP1 Proteostasis. Cell Rep. 2018 Jun
Subcellular Location
Cytoplasm.
Synonyms
Ubiquitin carboxyl-terminal hydrolase isozyme L3 antibody
Ubiquitin thioesterase L3 antibody
Ubiquitin thiolesterase antibody
Ubiquitin thiolesterase L3 antibody
UCH L3 antibody
UCH-L3 antibody
UCHL3 antibody
UCHL3_HUMAN antibody
Images
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Western blot analysis of UCHL3 on different lysates with Rabbit anti-UCHL3 antibody (ET7111-28) at 1/1,000 dilution.
Lane 1: Daudi cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: Neuro-2a cell lysate (20 µg/Lane)
Lane 4: PC-12 cell lysate (20 µg/Lane)
Lane 5: Mouse colon tissue lysate (40 µg/Lane)
Lane 6: Rat heart tissue lysate (40 µg/Lane)
Lane 7: Rat testis tissue lysate (40 µg/Lane)
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-28) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of UCHL3 on different lysates with Rabbit anti-UCHL3 antibody (ET7111-28) at 1/5,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-UCHL3 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 180 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-28) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"