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DPYD Recombinant Rabbit Monoclonal Antibody [JE54-79]

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Specification

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Catalog# ET7110-83

DPYD Recombinant Rabbit Monoclonal Antibody [JE54-79]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

DPYD Recombinant Rabbit Monoclonal Antibody [JE54-79]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human DPYD aa 831-970 / 1,025.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

111 kDa

Positive Control

THP-1 cell lysates, SiHa cell lysates, human liver tissue.

Conjugation

unconjugated

Clone Number

JE54-79

RRID

AB_3071043

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

Target

Function

The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Mutations in this gene result in dihydropyrimidine dehydrogenase deficiency, an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy. Two transcript variants encoding different isoforms have been found for this gene. In humans the enzyme is encoded by the DPYD gene. It is the initial and rate-limiting step in pyrimidine catabolism.[citation needed] It catalyzes the reduction of uracil and thymine. It is also involved in the degradation of the chemotherapeutic drugs 5-fluorouracil and tegafur. It also participates in beta-alanine metabolism and pantothenate and coa biosynthesis. The protein is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Genetic deficiency of this enzyme results in an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy.

Background References

1. Henricks LM. et. al. DPYD genotype-guided dose individualisation of fluoropyrimidine therapy in patients with cancer: a prospective safety analysis. Lancet Oncol. 2018 Nov

2. Wigle TJ. et. al. DPYD and Fluorouracil-Based Chemotherapy: Mini Review and Case Report. Pharmaceutics. 2019 May

Sequence Similarity

Belongs to the dihydropyrimidine dehydrogenase family.

Tissue Specificity

Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q12882 Human

Synonyms

DHP antibody

DHPDHase antibody

Dihydropyrimidine dehydrogenase [NADP(+)] antibody

Dihydropyrimidine dehydrogenase [NADP+] antibody

Dihydropyrimidine dehydrogenase antibody

Dihydrothymine dehydrogenase antibody

Dihydrouracil dehydrogenase antibody

DPD antibody

DPYD antibody

DPYD_HUMAN antibody

Expand

Images

  • Western blot analysis of DPYD on THP-1 cell lysates with Rabbit anti-DPYD antibody (<a href="/products/ET7110-83" style="font-weight: bold;text-decoration: underline;">ET7110-83</a>) at 1/500 dilution.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 111 kDa<br />Observed band size: 111 kDa<br /><br />Exposure time: 2 minutes;<br />6% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7110-83" style="font-weight: bold;text-decoration: underline;">ET7110-83</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of DPYD on THP-1 cell lysates with Rabbit anti-DPYD antibody (ET7110-83) at 1/500 dilution.

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 111 kDa
    Observed band size: 111 kDa

    Exposure time: 2 minutes;
    6% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-83) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/5,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of DPYD on SiHa cell lysates with Rabbit anti-DPYD antibody (<a href="/products/ET7110-83" style="font-weight: bold;text-decoration: underline;">ET7110-83</a>) at 1/500 dilution.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 111 kDa<br />Observed band size: 111 kDa<br /><br />Exposure time: 2 minutes;<br />6% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7110-83" style="font-weight: bold;text-decoration: underline;">ET7110-83</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of DPYD on SiHa cell lysates with Rabbit anti-DPYD antibody (ET7110-83) at 1/500 dilution.

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 111 kDa
    Observed band size: 111 kDa

    Exposure time: 2 minutes;
    6% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-83) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/5,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-DPYD antibody (<a href="/products/ET7110-83" style="font-weight: bold;text-decoration: underline;">ET7110-83</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7110-83" style="font-weight: bold;text-decoration: underline;">ET7110-83</a>) at 1/200 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-DPYD antibody (ET7110-83) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-83) at 1/200 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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