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NEK6 Recombinant Rabbit Monoclonal Antibody [JE51-72]

Usd: 385

Specification

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Catalog# ET7110-15

NEK6 Recombinant Rabbit Monoclonal Antibody [JE51-72]

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

NEK6 Recombinant Rabbit Monoclonal Antibody [JE51-72]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human NEK6 aa 170-313 / 313.

Species Reactivity

Human

Validated Applications

WB

Target Molecular Weight

Predicted band size: 36 kDa

Positive Control

THP-1 cell lysate, Wild-type 293T whole cell lysate.

Conjugation

unconjugated

Clone Number

JE51-72

RRID

AB_3070982

Product Features

Form

Liquid

Concentration

1 mg/mL(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

Target

Function

Serine/threonine-protein kinase Nek6 is an enzyme that in humans is encoded by the NEK6 gene. The Aspergillus nidulans 'never in mitosis A' (NIMA) gene encodes a serine/threonine kinase that controls initiation of mitosis. NIMA-related kinases (NEKs) are a group of protein kinases that are homologous to NIMA. Evidence suggests that NEKs perform functions similar to those of NIMA. It is a protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4, STAT3 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence.

Background References

1. Jee H.J. et. al. Nek6 overexpression antagonizes p53-induced senescence in human cancer cells. Cell Cycle 9:4703-4710(2010).

2. Vaz Meirelles G. et. al. Characterization of hNek6 interactome reveals an important role for its short N-terminal domain and colocalization with proteins at the centrosome. J. Proteome Res. 9:6298-6316(2010).

Sequence Similarity

Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.

Tissue Specificity

Ubiquitous, with highest expression in heart and skeletal muscle.

Post-translational Modification

Autophosphorylated. Phosphorylation at Ser-206 is required for its activation. Phosphorylated upon IR or UV-induced DNA damage. Phosphorylated by CHEK1 and CHEK2. Interaction with APBB1 down-regulates phosphorylation at Thr-210.

Subcellular Location

Cytoplasm, Cytoskeleton, Microtubule, Nucleus.

UNIPROT

SwissProt: Q9HC98 Human

Synonyms

Highly similar to cell cycle protein kinase CDC5/MSD2 antibody

NEK6 antibody

NEK6_HUMAN antibody

Never in mitosis A-related kinase 6 antibody

NIMA (Never In Mitosis Gene A) Related Kinase 6 antibody

NimA related protein kinase 6 antibody

NimA-related protein kinase 6 antibody

Protein kinase SID6 1512 antibody

Protein kinase SID6-1512 antibody

Putative serine threonine protein kinase antibody

Expand

Images

  • Western blot analysis of NEK6 on different lysates with Rabbit anti-NEK6 antibody (<a href="/products/ET7110-15" style="font-weight: bold;text-decoration: underline;">ET7110-15</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa (Human cervical adenocarcinoma cell) (15 µg/Lane)<br />Lane 2: THP-1 (Human acute monoblastic leukemia cell) (15 µg/Lane)<br />Lane 3: Hep G2 (Human hepatocellular carcinoma cell) (15 µg/Lane)<br />Lane 4: HEK-293 (Human embryonic kidney cell) (15 µg/Lane)<br />Lane 5: Mouse brain tissue lysate (30 µg/Lane)<br />Lane 6: Rat brain tissue lysate (30 µg/Lane)<br /> <br />Exposure time: 180 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET7110-15" style="font-weight: bold;text-decoration: underline;">ET7110-15</a>, 1/2,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 36 kDa<br />Observed band size: 36 kDa

    Western blot analysis of NEK6 on different lysates with Rabbit anti-NEK6 antibody (ET7110-15) at 1/2,000 dilution.

    Lane 1: HeLa (Human cervical adenocarcinoma cell) (15 µg/Lane)
    Lane 2: THP-1 (Human acute monoblastic leukemia cell) (15 µg/Lane)
    Lane 3: Hep G2 (Human hepatocellular carcinoma cell) (15 µg/Lane)
    Lane 4: HEK-293 (Human embryonic kidney cell) (15 µg/Lane)
    Lane 5: Mouse brain tissue lysate (30 µg/Lane)
    Lane 6: Rat brain tissue lysate (30 µg/Lane)

    Exposure time: 180 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET7110-15, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 36 kDa
    Observed band size: 36 kDa

  • <span style="font-weight: bold;">☑ Knockout (KO)</span><br /><br />All lanes: Western blot analysis of NEK6 with anti-NEK6 antibody[JE51-72] (<a href="/products/ET7110-15" style="font-weight: bold;text-decoration: underline;">ET7110-15</a>) at 1/1,000 dilution.<br />Lane 1: Wild-type 293T whole cell lysate (30 µg).<br />Lane 2: NEK6 knockout 293T whole cell lysate (30 µg).<br /><br /><a href="/products/ET7110-15" style="font-weight: bold;text-decoration: underline;">ET7110-15</a> was shown to specifically react with NEK6 in wild-type 293T cells. No band was observed when NEK6 knockout sample was tested. Wild-type and NEK6 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7110-15" style="font-weight: bold;text-decoration: underline;">ET7110-15</a>, 1/1,000) was used in 5% BSA at room temperature for 2 hours.

    ☑ Knockout (KO)

    All lanes: Western blot analysis of NEK6 with anti-NEK6 antibody[JE51-72] (ET7110-15) at 1/1,000 dilution.
    Lane 1: Wild-type 293T whole cell lysate (30 µg).
    Lane 2: NEK6 knockout 293T whole cell lysate (30 µg).

    ET7110-15 was shown to specifically react with NEK6 in wild-type 293T cells. No band was observed when NEK6 knockout sample was tested. Wild-type and NEK6 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET7110-15, 1/1,000) was used in 5% BSA at room temperature for 2 hours.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"