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Tryptophanyl tRNA synthetase / WRS Recombinant Rabbit Monoclonal Antibody [JE48-08]

Usd: 385

Specification

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Catalog# ET7109-73

Tryptophanyl tRNA synthetase / WRS Recombinant Rabbit Monoclonal Antibody [JE48-08]

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Tryptophanyl tRNA synthetase / WRS Recombinant Rabbit Monoclonal Antibody [JE48-08]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Tryptophanyl tRNA synthetase aa 385-471 / 471.

Species Reactivity

Human

Validated Applications

WB, IHC-P, FC

Target Molecular Weight

Predicted band size: 53 kDa

Positive Control

HeLa cell lysates, human lung tissue, A549.

Conjugation

unconjugated

Clone Number

JE48-08

RRID

AB_3070949

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene.

Background References

1. Wakasugi K et al. A human aminoacyl-tRNA synthetase as a regulator of angiogenesis. Proc. Natl. Acad. Sci. U.S.A. 99:173-177 (2002).

2. Bange FC et al. An interferon-induced protein with release factor activity is a tryptophanyl-tRNA synthetase. FEBS Lett 300:162-166 (1992).

Sequence Similarity

Belongs to the class-I aminoacyl-tRNA synthetase family.

Post-translational Modification

Proteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P23381 Human

Synonyms

Gamma 2 antibody

GAMMA-2 antibody

Gamma2 antibody

hWRS antibody

IFI 53 antibody

IFI53 antibody

IFP 53 antibody

IFP53 antibody

Interferon induced protein 53 antibody

Interferon-induced protein 53 antibody

Expand

Images

  • Western blot analysis of Tryptophanyl tRNA synthetase / WRS on HeLa cell lysate with Rabbit anti-Tryptophanyl tRNA synthetase / WRS antibody (<a href="/products/ET7109-73" style="font-weight: bold;text-decoration: underline;">ET7109-73</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 30 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET7109-73" style="font-weight: bold;text-decoration: underline;">ET7109-73</a>, 1/1,000 in 5% NFDM/TBST, room temperature for 2 hours<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 53 kDa<br />Observed band size: 53 kDa

    Western blot analysis of Tryptophanyl tRNA synthetase / WRS on HeLa cell lysate with Rabbit anti-Tryptophanyl tRNA synthetase / WRS antibody (ET7109-73) at 1/1,000 dilution.

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 30 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET7109-73, 1/1,000 in 5% NFDM/TBST, room temperature for 2 hours
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 53 kDa
    Observed band size: 53 kDa

  • Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Tryptophanyl tRNA synthetase / WRS antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7109-73" style="font-weight: bold;text-decoration: underline;">ET7109-73</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Tryptophanyl tRNA synthetase / WRS antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-73, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of Tryptophanyl tRNA synthetase / WRS was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ET7109-73" style="font-weight: bold;text-decoration: underline;">ET7109-73</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Tryptophanyl tRNA synthetase / WRS was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7109-73, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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