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Fbx32 Recombinant Rabbit Monoclonal Antibody [JE41-27]

Usd: 385

Specification

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Catalog# ET7109-25

Fbx32 Recombinant Rabbit Monoclonal Antibody [JE41-27]

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Fbx32 Recombinant Rabbit Monoclonal Antibody [JE41-27]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Fbx32 aa 1-160 / 355.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 41 kDa

Positive Control

Mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, A431, L6.

Conjugation

unconjugated

Clone Number

JE41-27

RRID

AB_3070914

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Cell

  • 1:50-1:200

Target

Function

Muscle atrophy F-box (MAFbx) is an E3 ubiquitin ligase that initiates ATP-dependent ubiquitin-mediated proteolysis and promotes muscle atrophy. MAFbx transcript is abundant in cardiac and skeletal muscle undergoing atrophy. MAFbx -/- mice are resistant to muscle atrophy. MAFbx is thought to recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation during skeletal muscle atrophy. It interacts with MyoD by ubiquitination via a sequence found in transcriptional coactivators and therefore may play an important role in the course of muscle differentiation by determining the abundance of MyoD. MAFbx is specifically expressed in cardiac and skeletal muscle.

Background References

1. Julie L C et al. Identification of essential sequences for cellular localization in the muscle-specific ubiquitin E3 ligase MAFbx/Atrogin 1. FEBS Lett 586:362-367 (2012).

2. Al-Hassnan Z N et al. A substitution mutation in cardiac ubiquitin ligase, FBXO32, is associated with an autosomal recessive form of dilated cardiomyopathy. BMC Med Genet 17:3-3 (2016).

Tissue Specificity

Specifically expressed in cardiac and skeletal muscle.

Subcellular Location

Cytoplasm. Nucleus.

UNIPROT

SwissProt: Q969P5 Human

SwissProt: Q9CPU7 Mouse

SwissProt: Q91Z62 Rat

Synonyms

4833442G10Rik antibody

AI430017 antibody

Atrogin 1 antibody

Atrogin-1 antibody

ATROGIN1 antibody

Atrophy gene 1 antibody

F box only protein 32 antibody

F-box only protein 32 antibody

F-box protein 32 antibody

FBX32_HUMAN antibody

Expand

Images

  • Western blot analysis of Fbx32 on different lysates with Rabbit anti-Fbx32 antibody (<a href="/products/ET7109-25" style="font-weight: bold;text-decoration: underline;">ET7109-25</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse skeletal muscle tissue lysate<br />Lane 2: Rat skeletal muscle tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 41 kDa<br />Observed band size: 41 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7109-25" style="font-weight: bold;text-decoration: underline;">ET7109-25</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Fbx32 on different lysates with Rabbit anti-Fbx32 antibody (ET7109-25) at 1/2,000 dilution.

    Lane 1: Mouse skeletal muscle tissue lysate
    Lane 2: Rat skeletal muscle tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 41 kDa
    Observed band size: 41 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-25) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • ICC staining of Fbx32 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ET7109-25" style="font-weight: bold;text-decoration: underline;">ET7109-25</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Fbx32 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7109-25, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • ICC staining of Fbx32 in L6 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ET7109-25" style="font-weight: bold;text-decoration: underline;">ET7109-25</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Fbx32 in L6 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7109-25, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation