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BAF57 Recombinant Rabbit Monoclonal Antibody [JE41-11]

Usd: 385

Specification

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Catalog# ET7109-23

BAF57 Recombinant Rabbit Monoclonal Antibody [JE41-11]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

BAF57 Recombinant Rabbit Monoclonal Antibody [JE41-11]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human BAF57 aa 1-139 / 411.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP

Target Molecular Weight

47 kDa

Positive Control

SH-SY-5Y, HepG2, SH-SY-5Y, SK-Br-3, rat uterus tissue, human gallbladder tissue, human lung cancer tissue, human colon tissue.

Conjugation

unconjugated

Clone Number

JE41-11

RRID

AB_3070912

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • IP

  • 1:50-1:200

Target

Function

The SWI/SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF1 or SNF2α) and Brg-1 (also designated SNF2 or SNF2β) are the ATPase subunits of the mammalian SWI/SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI/SNF complex. In higher eukaryotes, BAF57 is also a critical component of the SWI/ SNF complex. BAF57 contains a high-mobility-group (HMG) domain adjacent to a kinesin-like region and is a DNA-binding subunit of the SWI/SNF complex. The human BAF57 gene maps within the q12-25 region of chromosome 17, a gene-rich area implicated in breast and ovarian cancers.

Background References

1. Martens J A et al. Recent advances in understanding chromatin remodeling by SWI/SNF complexes. Curr Opin Genet Dev 13:136-142 (2003).

2. Euskirchen G et al. SWI/SNF chromatin-remodeling factors: multiscale analyses and diverse functions. J Biol Chem 287:30897-30905 (2012).

Post-translational Modification

Ubiquitinated by TRIP12, leading to its degradation by the proteasome. Ubiquitination is prevented upon interaction between TRIP12 and SMARCC1.

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q969G3 Human

SwissProt: O54941 Mouse

SwissProt: Q56A18 Rat

Synonyms

BAF57 antibody

BRG1 associated factor 57 antibody

BRG1-associated factor 57 antibody

Chromatin remodeling complex BRG1 associated factor 57 antibody

FLJ35648 antibody

SMARCE 1 antibody

SMARCE1 antibody

SMCE1_HUMAN antibody

SWI/SNF related matrix associated actin dependent regulator of chromatin e1 antibody

SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily e member 1 antibody

Expand

Images

  • ICC staining BAF57 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining BAF57 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining BAF57 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining BAF57 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining BAF57 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining BAF57 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-BAF57 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-BAF57 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using anti-BAF57 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using anti-BAF57 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-BAF57/SMARCE1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-BAF57/SMARCE1 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-BAF57 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-BAF57 antibody. Counter stained with hematoxylin.

  • Western blot analysis of BAF57 on different lysates with Rabbit anti-BAF57 antibody (<a href="/products/ET7109-23" style="font-weight: bold;text-decoration: underline;">ET7109-23</a>) at 1/1,000 dilution.<br /><br />Lane 1: MCF-7 (Human breast cancer cell) cell lysate<br />Lane 2: Hela (Human cervical adenocarcinoma cell) cell lysate<br /><br /><br />Lysates/proteins at 10 µg/Lane.<br />Exposure time: 25s seconds; ECL: K1801<br /><br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET7109-23" style="font-weight: bold;text-decoration: underline;">ET7109-23</a>, 1/1,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 47 kDa<br />Observed band size: 55 kDa

    Western blot analysis of BAF57 on different lysates with Rabbit anti-BAF57 antibody (ET7109-23) at 1/1,000 dilution.

    Lane 1: MCF-7 (Human breast cancer cell) cell lysate
    Lane 2: Hela (Human cervical adenocarcinoma cell) cell lysate


    Lysates/proteins at 10 µg/Lane.
    Exposure time: 25s seconds; ECL: K1801


    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET7109-23, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 47 kDa
    Observed band size: 55 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"