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NXF1 Recombinant Rabbit Monoclonal Antibody [JE40-63]

Usd: 385

Specification

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Catalog# ET7109-12

NXF1 Recombinant Rabbit Monoclonal Antibody [JE40-63]

Application

  • WB

  • IF-Cell

  • IHC-P

  • IP

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

NXF1 Recombinant Rabbit Monoclonal Antibody [JE40-63]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human NXF1 aa 1-132 / 619.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IHC-P, IP

Target Molecular Weight

Predicted band size: 70 kDa

Positive Control

293 cell lysates, SiHa cell lysates, HEK-293, human brain tissue, human colon tissue.

Conjugation

unconjugated

Clone Number

JE40-63

RRID

AB_3070902

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IF-Cell

  • 1:100

  • IHC-P

  • 1:200

  • IP

  • 1:10-1:50

Target

Function

The constitutive transport element (CTE) of type D retroviruses serves as a signal of nuclear export for unspliced viral RNAs. TAP (tip-associating protein, also known as NXF1) mediates the export of CTE-containing simian type D retroviral RNAs through binding directly to the CTE. TAP is associated with a recognized mRNA export pathway and is a member of the multigene family of NXF proteins. NXF proteins belong to an evolutionarily conserved family of proteins, which are characterized by a leucine-rich-repeat domain (LRR) followed by a region known as the nuclear transport factor 2 (NTF2)-like domain.

Background References

1. Grueter P et al. TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus. Mol Cell 1:649-659 (1998).

2. Tang H et al. Specific interaction between RNA helicase A and Tap, two cellular proteins that bind to the constitutive transport element of type D retrovirus. J Biol Chem 275:32694-32700 (2000).

Sequence Similarity

Belongs to the NXF family.

Tissue Specificity

Expressed ubiquitously.

Subcellular Location

Nucleus. Cytoplasm.

UNIPROT

SwissProt: Q9UBU9 Human

Synonyms

DKFZp667O0311 antibody

DmNXF1 antibody

MEX67 antibody

MEX67, yeast, homolog of antibody

Mex67p antibody

mRNA export factor TAP antibody

Mvb1 antibody

Nuclear RNA export factor 1 antibody

Nuclear RNA export factor 1 homolog (S. cerevisiae) antibody

nxf 1 antibody

Expand

Images

  • Immunocytochemistry analysis of HEK-293 cells labeling NXF1 with Rabbit anti-NXF1 antibody (<a href="/products/ET7109-12" style="font-weight: bold;text-decoration: underline;">ET7109-12</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NXF1 antibody (<a href="/products/ET7109-12" style="font-weight: bold;text-decoration: underline;">ET7109-12</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HEK-293 cells labeling NXF1 with Rabbit anti-NXF1 antibody (ET7109-12) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NXF1 antibody (ET7109-12) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-NXF1 antibody (<a href="/products/ET7109-12" style="font-weight: bold;text-decoration: underline;">ET7109-12</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7109-12" style="font-weight: bold;text-decoration: underline;">ET7109-12</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-NXF1 antibody (ET7109-12) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-12) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-NXF1 antibody (<a href="/products/ET7109-12" style="font-weight: bold;text-decoration: underline;">ET7109-12</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7109-12" style="font-weight: bold;text-decoration: underline;">ET7109-12</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-NXF1 antibody (ET7109-12) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-12) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of NXF1 on different lysates with Rabbit anti-NXF1 antibody (<a href="/products/ET7109-12" style="font-weight: bold;text-decoration: underline;">ET7109-12</a>) at 1/2,000 dilution.<br /><br />Lane 1: HEK-293 (Human embryonic kidney cell) cell lysate<br /> <br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 180 seconds; ECL: K1801<br /><br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET7109-12" style="font-weight: bold;text-decoration: underline;">ET7109-12</a>, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 70 kDa<br />Observed band size: 70 kDa

    Western blot analysis of NXF1 on different lysates with Rabbit anti-NXF1 antibody (ET7109-12) at 1/2,000 dilution.

    Lane 1: HEK-293 (Human embryonic kidney cell) cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 180 seconds; ECL: K1801


    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET7109-12, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 70 kDa
    Observed band size: 70 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"