VAMP1 Recombinant Rabbit Monoclonal Antibody [JG40-19]
Catalog# ET7109-03
VAMP1 Recombinant Rabbit Monoclonal Antibody [JG40-19]
-
WB
-
IHC-P
-
IF-Cell
-
IF-Tissue
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
VAMP1 Recombinant Rabbit Monoclonal Antibody [JG40-19]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant full length protein of human VAMP1.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IF-Cell, IF-Tissue
Molecular Weight
Predicted band size: 13 kDa
Positive Control
Mouse cerebellum tissue lysate, rat brain tissue lysate, 293T, Hela, SH-SY-5Y, human prostate cancer tissue, mouse brain tissue.
Conjugation
unconjugated
Clone Number
JG40-19
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500-1:2,000
-
IF-Cell
-
1:50-1:200
-
IF-Tissue
-
1:50-1:200
-
IHC-P
-
1:50-1:200
Target
Function
Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP1 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Multiple alternative splice variants that encode proteins with alternative carboxy ends have been described, but the full-length nature of some variants has not been defined.
Background References
1. Bourassa C V et al. VAMP1 mutation causes dominant hereditary spastic ataxia in Newfoundland families. Am J Hum Genet 91:548-552 (2012).
2. Bechtel S et al. The full-ORF clone resource of the German cDNA consortium. BMC Genomics 8:399-399 (2007).
Sequence Similarity
Belongs to the synaptobrevin family.
Tissue Specificity
Nervous system, skeletal muscle and adipose tissue.
Post-translational Modification
(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type B (BoNT/B, botB) which probably hydrolyzes the 78-Gln-|-Phe-79 bond and inhibits neurotransmitter release.; (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type D (BoNT/D, botD) which probably hydrolyzes the 61-Arg-|-Leu-62 bond and inhibits neurotransmitter release. BoNT/D has low catalytic activity on this protein due to its sequence. Note that humans are not known to be infected by C.botulinum type D.; (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type F (BoNT/F, botF) which probably hydrolyzes the 60-Gln-|-Lys-61 bond and inhibits neurotransmitter release.; (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type X (BoNT/X) which probably hydrolyzes the 68-Arg-|-Ala-69 bond and inhibits neurotransmitter release. It remains unknown whether BoNT/X is ever produced, or what organisms it targets.
Subcellular Location
Mitochondrion. Synapse. Synaptosome.
Synonyms
DKFZp686H12131 antibody
SYB 1 antibody
SYB1 antibody
Synaptobrevin 1 antibody
Synaptobrevin-1 antibody
Synaptobrevin1 antibody
VAMP 1 antibody
VAMP-1 antibody
Vamp1 antibody
VAMP1_HUMAN antibody
ExpandImages
-
Western blot analysis of VAMP1 on different lysates with Rabbit anti-VAMP1 antibody (ET7109-03) at 1/500 dilution.
Lane 1: Mouse cerebellum tissue lysate
Lane 2: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 13 kDa
Observed band size: 15 kDa
Exposure time: 2 minutes;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-03) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
ICC staining VAMP1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
ICC staining VAMP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
ICC staining VAMP1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-VAMP1 antibody. Counter stained with hematoxylin.
-
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-VAMP1 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"