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VAMP1 Recombinant Rabbit Monoclonal Antibody [JG40-19]

Usd: 385

Specification

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Catalog# ET7109-03

VAMP1 Recombinant Rabbit Monoclonal Antibody [JG40-19]

Application

  • WB

  • IHC-P

  • IF-Cell

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

VAMP1 Recombinant Rabbit Monoclonal Antibody [JG40-19]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant full length protein of human VAMP1.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Cell, IF-Tissue

Target Molecular Weight

Predicted band size: 13 kDa

Positive Control

Mouse cerebellum tissue lysate, rat brain tissue lysate, 293T, Hela, SH-SY-5Y, human prostate cancer tissue, mouse brain tissue.

Conjugation

unconjugated

Clone Number

JG40-19

RRID

AB_3070893

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:20,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

Target

Function

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP1 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Multiple alternative splice variants that encode proteins with alternative carboxy ends have been described, but the full-length nature of some variants has not been defined.

Background References

1. Bourassa C V et al. VAMP1 mutation causes dominant hereditary spastic ataxia in Newfoundland families. Am J Hum Genet 91:548-552 (2012).

2. Bechtel S et al. The full-ORF clone resource of the German cDNA consortium. BMC Genomics 8:399-399 (2007).

Sequence Similarity

Belongs to the synaptobrevin family.

Tissue Specificity

Nervous system, skeletal muscle and adipose tissue.

Post-translational Modification

(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type B (BoNT/B, botB) which probably hydrolyzes the 78-Gln-|-Phe-79 bond and inhibits neurotransmitter release.; (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type D (BoNT/D, botD) which probably hydrolyzes the 61-Arg-|-Leu-62 bond and inhibits neurotransmitter release. BoNT/D has low catalytic activity on this protein due to its sequence. Note that humans are not known to be infected by C.botulinum type D.; (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type F (BoNT/F, botF) which probably hydrolyzes the 60-Gln-|-Lys-61 bond and inhibits neurotransmitter release.; (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type X (BoNT/X) which probably hydrolyzes the 68-Arg-|-Ala-69 bond and inhibits neurotransmitter release. It remains unknown whether BoNT/X is ever produced, or what organisms it targets.

Subcellular Location

Mitochondrion. Synapse. Synaptosome.

UNIPROT

SwissProt: P23763 Human

SwissProt: Q62442 Mouse

SwissProt: Q63666 Rat

Synonyms

DKFZp686H12131 antibody

SYB 1 antibody

SYB1 antibody

Synaptobrevin 1 antibody

Synaptobrevin-1 antibody

Synaptobrevin1 antibody

VAMP 1 antibody

VAMP-1 antibody

Vamp1 antibody

VAMP1_HUMAN antibody

Expand

Images

  • Western blot analysis of VAMP1 on different lysates with Rabbit anti-VAMP1 antibody (<a href="/products/ET7109-03" style="font-weight: bold;text-decoration: underline;">ET7109-03</a>) at 1/20,000 dilution.<br /><br />Lane 1: mouse brain tissue lysate <br />Lane 2: rat brain tissue lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br />Exposure time: 59 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET7109-03" style="font-weight: bold;text-decoration: underline;">ET7109-03</a>, 1/20,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 13 kDa<br />Observed band size: 16 kDa

    Western blot analysis of VAMP1 on different lysates with Rabbit anti-VAMP1 antibody (ET7109-03) at 1/20,000 dilution.

    Lane 1: mouse brain tissue lysate
    Lane 2: rat brain tissue lysate

    Lysates/proteins at 30 µg/Lane.
    Exposure time: 59 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET7109-03, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 13 kDa
    Observed band size: 16 kDa

  • ICC staining VAMP1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining VAMP1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining VAMP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining VAMP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining VAMP1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining VAMP1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-VAMP1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-VAMP1 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-VAMP1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-VAMP1 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"