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Monoamine Oxidase B Recombinant Rabbit Monoclonal Antibody [JG39-97]

Usd: 385

Specification

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Catalog# ET7108-98

Monoamine Oxidase B Recombinant Rabbit Monoclonal Antibody [JG39-97]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Monoamine Oxidase B Recombinant Rabbit Monoclonal Antibody [JG39-97]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Monoamine Oxidase B aa 1-126 / 520.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Target Molecular Weight

59 kDa

Positive Control

Human liver tissue lysate, SiHa cell lysate, human liver tissue, human prostate cancer tissue, human kidney tissue.

Conjugation

unconjugated

Clone Number

JG39-97

RRID

AB_3070889

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500

  • IHC-P

  • 1:50-1:500

Target

Function

Monoamine oxidase (MAO) is an enzyme of the mitochondrial outer membrane and catalyzes the oxidative deamination of biogenic amines throughout the body. MAO is critical in the neuronal metabolism of catecholamine and indolamine transmitters. Cultured skin fibroblasts show both MAO-A and MAO-B and both MAOs differ in molecular structure. MAO-A, the primary type in fibroblasts, preferentially degrades serotonin and norepinephrine. Only MAO-B is present in platelets and only MAO-A is present in trophoblasts. MAO-B, the primary type found not only in platelets but also in the brain of man and other primates, preferentially degrades phenylethylamine and benzylamine. MAO has been of particular interest to psychiatry and genetics because of the suggestion that low activity is a 'genetic marker' for schizophrenia. The genes which encode MAO-A and MAO-B map to human chromosome Xp11.23.

Background References

1. Wu H F et al. Site-directed mutagenesis of monoamine oxidase A and B: role of cysteines. Mol Pharmacol 43:888-893 (1993).

2. Cesura A M et al. Investigation on the structure of the active site of monoamine oxidase-B by affinity labeling with the selective inhibitor lazabemide and by site-directed mutagenesis. Eur J Biochem 236:996-1002 (1996).

Sequence Similarity

Belongs to the flavin monoamine oxidase family.

Subcellular Location

Mitochondrion.

UNIPROT

SwissProt: P27338 Human

Synonyms

Adrenalin oxidase antibody

Amine oxidase (flavin containing) antibody

Amine oxidase [flavin-containing] B antibody

AOFB_HUMAN antibody

MAO, brain antibody

MAO, platelet antibody

MAO-B antibody

MAOB antibody

MGC26382 antibody

Monoamine oxidase B antibody

Expand

Images

  • Western blot analysis of Monoamine Oxidase B on different lysates with Rabbit anti-Monoamine Oxidase B antibody (<a href="/products/ET7108-98" style="font-weight: bold;text-decoration: underline;">ET7108-98</a>) at 1/500 dilution.<br /><br />Lane 1: Human liver tissue lysate (20 µg/Lane)<br />Lane 2: SiHa cell lysate (10 µg/Lane)<br /><br />Predicted band size: 59 kDa<br />Observed band size: 59 kDa<br /><br />Exposure time: 2 minutes;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7108-98" style="font-weight: bold;text-decoration: underline;">ET7108-98</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Monoamine Oxidase B on different lysates with Rabbit anti-Monoamine Oxidase B antibody (ET7108-98) at 1/500 dilution.

    Lane 1: Human liver tissue lysate (20 µg/Lane)
    Lane 2: SiHa cell lysate (10 µg/Lane)

    Predicted band size: 59 kDa
    Observed band size: 59 kDa

    Exposure time: 2 minutes;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-98) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-Monoamine Oxidase B antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-Monoamine Oxidase B antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Monoamine Oxidase B antibody (<a href="/products/ET7108-98" style="font-weight: bold;text-decoration: underline;">ET7108-98</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-98" style="font-weight: bold;text-decoration: underline;">ET7108-98</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Monoamine Oxidase B antibody (ET7108-98) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-98) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Monoamine Oxidase B antibody (<a href="/products/ET7108-98" style="font-weight: bold;text-decoration: underline;">ET7108-98</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-98" style="font-weight: bold;text-decoration: underline;">ET7108-98</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Monoamine Oxidase B antibody (ET7108-98) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-98) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"