NOXA2 Recombinant Rabbit Monoclonal Antibody [JG86-31]
Catalog# ET7108-72
NOXA2 Recombinant Rabbit Monoclonal Antibody [JG86-31]
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WB
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IHC-P
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IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
NOXA2 Recombinant Rabbit Monoclonal Antibody [JG86-31]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human NOXA2 aa 180-320 / 526.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IP
Target Molecular Weight
60 kDa
Positive Control
Rat spleen tissue lysates, THP-1 cell lysate, rat spleen tissue, human spleen tissue, mouse spleen tissue.
Conjugation
unconjugated
Clone Number
JG86-31
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IHC-P
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1:50-1:200
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IP
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1:10-1:50
Target
Function
The heredity disease chronic granulomatous disease (CGF) has been linked to mutations in p47phox and p67phox. The cytosolic proteins p47phox and p67phox, also designated neutrophil cytosol factor 1(NCF1) and NCF2, respectively, are required for activation of the superoxide-producing NADPH oxidase in neutrophils and other phagocytic cells. During activation of the NADPH oxidase, p47phox and p67phox migrate to the plasma membrane where they associate with cytochrome b558 and the small G protein Rac to form the functional enzyme complex. Both p47phox and p67phox contain two Src homology 3 (SH3) domains. The C-terminal SH3 domain of p67phox has been shown to interact with the proline rich domain of p47phox, suggesting that p47phox may faciliate the transport of p67phox to the membrane.
Background References
1. Yoshida L S et al. Expression of a p67(phox) homolog in Caco-2 cells giving O(2)(-)-reconstituting ability to cytochrome b(558) together with recombinant p47(phox). Biochem Biophys Res Commun 296:1322-1328 (2002).
2. de Boer M et al. Autosomal recessive chronic granulomatous disease with absence of the 67-kD cytosolic NADPH oxidase component: identification of mutation and detection of carriers. Blood 83:531-536 (1994).
Sequence Similarity
Belongs to the NCF2/NOXA1 family.
Subcellular Location
Cytoplasm.
Synonyms
67 kDa neutrophil oxidase factor antibody
Chronic granulomatous disease autosomal 2 antibody
FLJ93058 antibody
NADPH oxidase activator 2 antibody
NCF-2 antibody
Ncf2 antibody
NCF2_HUMAN antibody
Neutrophil cytosol factor 2 antibody
Neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2) antibody
Neutrophil NADPH oxidase factor 2 antibody
ExpandImages
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Western blot analysis of NOXA2 on different lysates with Rabbit anti-NOXA2 antibody (ET7108-72) at 1/5,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: rat spleen tissue lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET7108-72, 1/5,000 in primary antibody dilution buffer (K1803), room temperature for 2 hours
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 60 kDa
Observed band size: 67 kDa -
Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-NOXA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-72, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NOXA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-72, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-NOXA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-72, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"