Gemin 1 Recombinant Rabbit Monoclonal Antibody [JG38-19]
Catalog# ET7108-63
Gemin 1 Recombinant Rabbit Monoclonal Antibody [JG38-19]
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WB
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IHC-P
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IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Gemin 1 Recombinant Rabbit Monoclonal Antibody [JG38-19]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Gemin aa 83-220 / 294.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IP
Target Molecular Weight
Predicted band size: 32 kDa
Positive Control
SiHa, rat testis tissue, mouse brain tissue.
Conjugation
unconjugated
Clone Number
JG38-19
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IHC-P
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1:50-1:200
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IP
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1:10-1:50
Target
Function
The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Ensures the correct splicing of U12 intron-containing genes that may be important for normal motor and proprioceptive neurons development. Also required for resolving RNA-DNA hybrids created by RNA polymerase II, that form R-loop in transcription terminal regions, an important step in proper transcription termination. May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs).
Background References
1. Yanling Zhao D. et al. SMN and symmetric arginine dimethylation of RNA polymerase II C-terminal domain control termination. Nature 529:48-53(2016).
2. Lotti F. et al. An SMN-dependent U12 splicing event essential for motor circuit function. Cell 151:440-454(2012).
Sequence Similarity
Belongs to the SMN family.
Tissue Specificity
Expressed in a wide variety of tissues. Expressed at high levels in brain, kidney and liver, moderate levels in skeletal and cardiac muscle, and low levels in fibroblasts and lymphocytes. Also seen at high levels in spinal cord. Present in osteoclasts and mononuclear cells (at protein level).
Subcellular Location
Nucleus.
UNIPROT
Synonyms
BCD541 antibody
Component of gems 1 antibody
Gemin 1 antibody
Gemin-1 antibody
OTTHUMP00000125198 antibody
OTTHUMP00000223567 antibody
OTTHUMP00000223568 antibody
OTTHUMP00000224066 antibody
OTTHUMP00000226924 antibody
SMA 1 antibody
ExpandImages
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Western blot analysis of Gemin 1 on SiHa cell lysate lysate with Rabbit anti-Gemin 1 antibody (ET7108-63) at 1/1,000 dilution.
Lysates/proteins at 15 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET7108-63, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 32 kDa
Observed band size: 35 kDa -
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-Gemin 1 antibody (ET7108-63) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-63) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Gemin 1 antibody (ET7108-63) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-63) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"