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PICK1 Recombinant Rabbit Monoclonal Antibody [JG30-80]

Usd: 385

Specification

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Catalog# ET7108-60

PICK1 Recombinant Rabbit Monoclonal Antibody [JG30-80]

Application

  • WB

  • IP

  • FC

Reactivity

  • Human

Predicted reactivity

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

PICK1 Recombinant Rabbit Monoclonal Antibody [JG30-80]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human PICK1 aa 1-50 / 415.

Species Reactivity

Human (Predicted: Mouse, Rat)

Predicted species support after-sales service

Validated Applications

WB, IP, FC

Target Molecular Weight

47 kDa

Positive Control

HeLa cell lysate, MCF7 cell lysate,293T cell lysate, LNCap cell lysate.

Conjugation

unconjugated

Clone Number

JG30-80

RRID

AB_3070857

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • FC

  • 1:50-1:100

  • IP

  • Use at an assay dependent concentration.

Target

Function

Protein interacting with C kinase 1 (PICK1) is a PDZ-domain containing protein that is located in the perinuclear region and is phosphorylated in response to PKCα activation. PKCα, which is essential for the regulation of proliferation and differentiation in numerous cell types, contains within its catalytic region a PDZ-binding domain that is absent from other PKC isoforms. Mutation of the PICK1 PDZ domain inhibits the binding of PICK1 to PKCα. PICK1 also interacts with the carboxy terminus of a-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid (AMPA) receptor, a neurotransmitter receptor located at excitatory synapses, suggesting that PICK1 plays a role in the modulation of synaptic transmission by targeting and anchoring AMPA to specific synapses.

Background References

1. Shao X et al. Threonine 82 at the PDZ domain of PICK1 is critical for AMPA receptor interaction and localization. Neurochem Int 56:962-970 (2010).

2. Takeya R et al. Interaction of the PDZ domain of human PICK1 with class I ADP-ribosylation factors. Biochem Biophys Res Commun 267:149-155 (2000).

Tissue Specificity

Ubiquitous.

Post-translational Modification

Phosphorylation at Thr-82 appears to inhibit the interaction with AMPA receptors.; Palmitoylation on Cys-413 is essential for long-term synaptic depression (LTD).

Subcellular Location

cytoskeleton, perinuclear region, membrane, postsynaptic density, synaptosome.

UNIPROT

SwissProt: Q9NRD5 Human

Synonyms

dJ1039K5 antibody

MGC15204 antibody

OTTHUMP00000028509 antibody

PICK 1 antibody

Pick1 antibody

PICK1_HUMAN antibody

PRKCA binding protein antibody

PRKCA-binding protein antibody

PRKCABP antibody

Protein interacting with C kinase 1 antibody

Expand

Images

  • Western blot analysis of PICK1 on different lysates with Rabbit anti-PICK1 antibody (<a href="/products/ET7108-60" style="font-weight: bold;text-decoration: underline;">ET7108-60</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate<br />Lane 2: MCF7 (Human breast cancer cell) cell lysate<br />Lane 3: 293T (Human embryonic kidney cell) cell lysate<br />Lane 4: LNCaP (Human prostate cancer cell) cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 10 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET7108-60" style="font-weight: bold;text-decoration: underline;">ET7108-60</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 47 kDa<br />Observed band size: 47 kDa

    Western blot analysis of PICK1 on different lysates with Rabbit anti-PICK1 antibody (ET7108-60) at 1/5,000 dilution.

    Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
    Lane 2: MCF7 (Human breast cancer cell) cell lysate
    Lane 3: 293T (Human embryonic kidney cell) cell lysate
    Lane 4: LNCaP (Human prostate cancer cell) cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 10 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET7108-60, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 47 kDa
    Observed band size: 47 kDa

  • Flow cytometric analysis of PICK1 was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ET7108-60" style="font-weight: bold;text-decoration: underline;">ET7108-60</a>, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).

    Flow cytometric analysis of PICK1 was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7108-60, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"