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SF3B3 Recombinant Rabbit Monoclonal Antibody [JG63-53]

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Specification

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Catalog# ET7108-36

SF3B3 Recombinant Rabbit Monoclonal Antibody [JG63-53]

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

SF3B3 Recombinant Rabbit Monoclonal Antibody [JG63-53]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human SF3B3 aa 1061-1217 / 1217.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, FC

Target Molecular Weight

136 kDa

Positive Control

Jurkat cell lysate, Hela cell lysate, human colon cancer tissue, human kidney tissue, mouse testis tissue, human tonsil tissue, rat brain tissue, LOVO.

Conjugation

unconjugated

Clone Number

JG63-53

RRID

AB_3070835

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IF-Cell

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

SF3B is a U2 snRNP-associated protein complex essential for spliceosome assembly. SF3B contains the spliceosomal proteins SAP 49, SAP 130 (also known as SF3B3), SAP 145 and SAP 155. SF3B3, SAP 145 and SAP 155 are present in a protein complex in HeLa nuclear extracts and associate with one another. While SF3B3 and SAP 155 interact with each other (directly or indirectly) within this complex, SAP 49 and SAP 145 are known to interact directly with each other. Unexpectedly, the SAP 49-SAP 145 protein-protein interaction requires the amino-terminus of SAP 49, which contains two RNA-recognition motifs. The observation that SAP 49 and SAP 145 interact directly with both U2 snRNP and the pre-mRNA suggests that this protein complex plays a role in tethering U2 snRNP to the branch site.

Background References

1. Das B K et al. Characterization of a protein complex containing spliceosomal proteins saps 49,130,145 and 155. Mol Cell Biol 19:6796-6802 (1999).

2. Das R et al. Functional association of U2 snRNP with the ATP-independent spliceosomal complex E. Mol Cell 5:779-787 (2000).

Sequence Similarity

Belongs to the RSE1 family.

Subcellular Location

Nucleus. Spliceosome.

UNIPROT

SwissProt: Q15393 Human

SwissProt: Q921M3 Mouse

Entrez Gene: 292019 Rat

Synonyms

KIAA0017 antibody

Pre mRNA splicing factor SF3b 130 kDa subunit antibody

Pre-mRNA-splicing factor SF3b 130 kDa subunit antibody

RSE1 antibody

SAP 130 antibody

SAP130 antibody

SF3b130 antibody

SF3B3 antibody

SF3B3_HUMAN antibody

Spliceosome associated protein 130 antibody

Expand

Images

  • Western blot analysis of SF3B3 on different lysates with Rabbit anti-SF3B3 antibody (<a href="/products/ET7108-36" style="font-weight: bold;text-decoration: underline;">ET7108-36</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa<br />Lane 2: Jurkat<br />Lane 3: RAW264.7 <br />Lane 4: C6<br />Lane 5: Mouse brain<br />Lane 6: Mouse kidney<br />Lane 7: Rat brain<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 20 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET7108-36" style="font-weight: bold;text-decoration: underline;">ET7108-36</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 135.6 kDa<br />Observed band size: 136 kDa

    Western blot analysis of SF3B3 on different lysates with Rabbit anti-SF3B3 antibody (ET7108-36) at 1/5,000 dilution.

    Lane 1: HeLa
    Lane 2: Jurkat
    Lane 3: RAW264.7
    Lane 4: C6
    Lane 5: Mouse brain
    Lane 6: Mouse kidney
    Lane 7: Rat brain

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 20 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET7108-36, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 135.6 kDa
    Observed band size: 136 kDa

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-SF3B3 antibody (<a href="/products/ET7108-36" style="font-weight: bold;text-decoration: underline;">ET7108-36</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-36" style="font-weight: bold;text-decoration: underline;">ET7108-36</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-SF3B3 antibody (ET7108-36) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-36) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SF3B3 antibody (<a href="/products/ET7108-36" style="font-weight: bold;text-decoration: underline;">ET7108-36</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-36" style="font-weight: bold;text-decoration: underline;">ET7108-36</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SF3B3 antibody (ET7108-36) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-36) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-SF3B3 antibody (<a href="/products/ET7108-36" style="font-weight: bold;text-decoration: underline;">ET7108-36</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-36" style="font-weight: bold;text-decoration: underline;">ET7108-36</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-SF3B3 antibody (ET7108-36) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-36) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-SF3B3 antibody (<a href="/products/ET7108-36" style="font-weight: bold;text-decoration: underline;">ET7108-36</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-36" style="font-weight: bold;text-decoration: underline;">ET7108-36</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-SF3B3 antibody (ET7108-36) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-36) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-SF3B3 antibody (<a href="/products/ET7108-36" style="font-weight: bold;text-decoration: underline;">ET7108-36</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-36" style="font-weight: bold;text-decoration: underline;">ET7108-36</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-SF3B3 antibody (ET7108-36) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-36) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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