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MBD3 Recombinant Rabbit Monoclonal Antibody [JB61-47]

Usd: 385

Specification

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Catalog# ET7107-78

MBD3 Recombinant Rabbit Monoclonal Antibody [JB61-47]

Application

  • WB

  • IHC-P

  • FC

  • IF-Cell

  • IF-Tissue

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

MBD3 Recombinant Rabbit Monoclonal Antibody [JB61-47]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human MBD3 aa 160-291 / 291.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, FC, IF-Cell, IF-Tissue, IP

Target Molecular Weight

Predicted band size: 33 kDa

Positive Control

MCF-7 cell lysate, A549, NIH/3T3, SH-SY-5Y, rat testis tissue, human placenta tissue, mouse brain tissue.

Conjugation

unconjugated

Clone Number

JB61-47

RRID

AB_3070786

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:50-1:500

  • FC

  • 1:50-1:100

  • IP

  • Use at an assay dependent concentration.

Target

Function

Methylation of DNA contributes to the regulation of gene transcription in both mammalian and invertebrate systems. DNA methylation predominates on cytosine residues that are present in dinucleotide motifs consisting of a 5′ cytosine followed by guanosine (CpG), and it requires the enzymatic activity of DNA methyltransferase, which results in transcriptional repression of the methylated gene. Several proteins have been identified that associate with the methyl-CpG sites, and they include methyl-CpG binding protein-1 (MBD1), MBD2, MBD3, MBD4 and MeCP2. Expression of the MBD proteins is highest in somatic tissues.

Background References

1. Hendrich B et al. Identification and characterization of a family of mammalian methyl-CpG binding proteins. Mol Cell Biol 18:6538-6547 (1998).

2. Tatematsu K et al. MBD2-MBD3 complex binds to hemi-methylated DNA and forms a complex containing DNMT1 at the replication foci in late S phase. Genes Cells 5:677-688 (2000).

Subcellular Location

Chromosome, Nucleus.

UNIPROT

SwissProt: O95983 Human

SwissProt: Q9Z2D8 Mouse

SwissProt: F7EY92 Rat

Synonyms

AI181826 antibody

AU019209 antibody

MBD 3 antibody

Mbd3 antibody

MBD3: methyl CpG binding domain protein 3 antibody

MBD3_HUMAN antibody

Methyl CpG binding domain protein 3 antibody

Methyl CpG binding protein MBD3 antibody

Methyl-CpG-binding domain protein 3 antibody

Methyl-CpG-binding protein MBD3 antibody

Images

  • Western blot analysis of MBD3 on different lysates with Rabbit anti-MBD3 antibody (<a href="/products/ET7107-78" style="font-weight: bold;text-decoration: underline;">ET7107-78</a>) at 1/5,000 dilution.<br /><br />Lane 1: MCF7 (Human breast cancer cell) cell lysate (15 µg/Lane)<br />Lane 2: U-937 (Human acute monocytic leukemia cell) cell lysate (15 µg/Lane)<br />Lane 3: HeLa (Human cervical adenocarcinoma cell) cell lysate (15 µg/Lane)<br />Lane 4: SH-SY5Y (Human neuroblastoma cell) cell lysate (15 µg/Lane)<br />Lane 5: Mouse brain tissue lysate (30 µg/Lane)<br />Lane 6: Rat brainc tissue lysate (30 µg/Lane)<br /><br />Exposure time: 25 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET7107-78" style="font-weight: bold;text-decoration: underline;">ET7107-78</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 33 kDa<br />Observed band size: 35 kDa

    Western blot analysis of MBD3 on different lysates with Rabbit anti-MBD3 antibody (ET7107-78) at 1/5,000 dilution.

    Lane 1: MCF7 (Human breast cancer cell) cell lysate (15 µg/Lane)
    Lane 2: U-937 (Human acute monocytic leukemia cell) cell lysate (15 µg/Lane)
    Lane 3: HeLa (Human cervical adenocarcinoma cell) cell lysate (15 µg/Lane)
    Lane 4: SH-SY5Y (Human neuroblastoma cell) cell lysate (15 µg/Lane)
    Lane 5: Mouse brain tissue lysate (30 µg/Lane)
    Lane 6: Rat brainc tissue lysate (30 µg/Lane)

    Exposure time: 25 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET7107-78, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 33 kDa
    Observed band size: 35 kDa

  • ICC staining MBD3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining MBD3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining MBD3 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining MBD3 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining MBD3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining MBD3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-MBD3 antibody (<a href="/products/ET7107-78" style="font-weight: bold;text-decoration: underline;">ET7107-78</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7107-78" style="font-weight: bold;text-decoration: underline;">ET7107-78</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-MBD3 antibody (ET7107-78) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-78) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-MBD3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-MBD3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-MBD3 antibody (<a href="/products/ET7107-78" style="font-weight: bold;text-decoration: underline;">ET7107-78</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7107-78" style="font-weight: bold;text-decoration: underline;">ET7107-78</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-MBD3 antibody (ET7107-78) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-78) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"