H Cadherin Recombinant Rabbit Monoclonal Antibody [JB14-48]
Catalog# ET7107-76
H Cadherin Recombinant Rabbit Monoclonal Antibody [JB14-48]
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IHC-P
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
H Cadherin Recombinant Rabbit Monoclonal Antibody [JB14-48]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human H Cadherin aa 81-220 / 713.
Species Reactivity
Human, Mouse, Rat
Validated Applications
IHC-P, WB
Target Molecular Weight
Predicted band size: 78 kDa
Positive Control
HaCaT cell lysate, human colon carcinoma tissue, human kidney tissue, rat skeletal muscle tissue, mouse heart tissue.
Conjugation
unconjugated
Clone Number
JB14-48
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:50-1:1,000
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WB
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1:1,000
Target
Function
Cadherins comprise a family of Ca++-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogen-esis. Cadherins each contain a large extracellular domains characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as β-catenin, to regulate cadherin function. T-cadherin (for truncated-cadherin), also designated heart-cadherin or cadherin-13) expression levels have been shown to be reduced in human breast cancers and carcinoma cell lines. Evidence suggests that decreased levels of T-cadherin indicate a progression in breast malignancies.
Background References
1. Takeuchi T et al. Expression of T-cadherin (CDH13, H-Cadherin) in human brain and its characteristics as a negative growth regulator of epidermal growth factor in neuroblastoma cells. J Neurochem 74:1489-1497 (2000).
2. Liu T et al. Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. J Proteome Res 4:2070-2080 (2005).
Tissue Specificity
Highly expressed in heart. In the CNS, expressed in cerebral cortex, medulla, hippocampus, amygdala, thalamus and substantia nigra. No expression detected in cerebellum or spinal cord.
Subcellular Location
Cell membrane, Membrane.
Synonyms
CAD13_HUMAN antibody
Cadherin-13 antibody
CDH13 antibody
CDHH antibody
H-cadherin antibody
Heart cadherin antibody
P105 antibody
T cad antibody
T Cadherin antibody
T-cad antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of H Cadherin on different lysates with Rabbit anti-H Cadherin antibody (ET7107-76) at 1/1,000 dilution.
Lane 1: HaCaT cell lysate
Lane 2: HaCaT cell lysate treated with deglycosylation
Lysates/proteins at 20 µg/Lane.
Predicted band size: 78 kDa
Observed band size: 75-130 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-76) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-H Cadherin antibody (ET7107-76) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-76) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-H Cadherin antibody (ET7107-76) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-76) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-H Cadherin antibody (ET7107-76) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-76) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-H Cadherin antibody (ET7107-76) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-76) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"