STIP1 Recombinant Rabbit Monoclonal Antibody [JB95-38]
Catalog# ET7107-63
STIP1 Recombinant Rabbit Monoclonal Antibody [JB95-38]
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WB
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IHC-P
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FC
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IF-Cell
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IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
STIP1 Recombinant Rabbit Monoclonal Antibody [JB95-38]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human STIP1 aa 1-137 / 543.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, FC, IF-Cell, IP
Target Molecular Weight
Predicted band size: 63 kDa
Positive Control
Mouse testis tissue lysate, rat testis tissue lysate, human colon cancer tissue, human spleen tissue, mouse testis tissue.
Conjugation
unconjugated
Clone Number
JB95-38
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
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IF-Cell
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1:50
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IP
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Use at an assay dependent concentration.
Target
Function
Stress-induced-phosphoprotein 1 (STI1) functions as a co-chaperone for HSP70 and HSP90 during heat shock response. STI1 exists as either a monomer or a dimer, and this conformational flexibility facilitates its function in organizing HSP70/HSP90. HSP90 acts as an ATPase, and requires the recruitment of client proteins and proper conformation to function. STI1 acts as a "scaffold" for client protein recruitment to the relaxed, ADP-bound conformation of HSP90, thus suppressing ATP turnover during the loading phase and allowing proper function.
Background References
1. Woodford M R et al. The FNIP co-chaperones decelerate the Hsp90 chaperone cycle and enhance drug binding. Nat Commun 7:12037-12037 (2016).
2. Silverstein A M et al. Protein phosphatase 5 is a major component of glucocorticoid receptor.hsp90 complexes with properties of an FK506-binding immunophilin. J Biol Chem 272:16224-16230 (1997).
Subcellular Location
Cytoplasm, Nucleus.
Synonyms
Epididymis secretory sperm binding protein Li 94n antibody
HEL S 94n antibody
Hop antibody
Hsc70/Hsp90 organizing protein antibody
Hsc70/Hsp90-organizing protein antibody
IEF SSP 3521 antibody
NY REN 11 antigen antibody
P60 antibody
Renal carcinoma antigen NY-REN-11 antibody
STI1 antibody
ExpandImages
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Western blot analysis of STIP1 on different lysates with Rabbit anti-STIP1 antibody (ET7107-63) at 1/1,000 dilution.
Lane 1: Mouse testis tissue lysate
Lane 2: Rat testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 5 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET7107-63, 1/1,000 in 5% NFDM/TBST, 2 hours at room temperature
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/100,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 63 kDa
Observed band size: 63 kDa -
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-STIP1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-STIP1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-STIP1 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"