DDAH2 Recombinant Rabbit Monoclonal Antibody [JB59-33]
Catalog# ET7107-41
DDAH2 Recombinant Rabbit Monoclonal Antibody [JB59-33]
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WB
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IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
DDAH2 Recombinant Rabbit Monoclonal Antibody [JB59-33]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human DDAH2 aa 1-125 / 285.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IP
Target Molecular Weight
Predicted band size: 30 kDa
Positive Control
MCF7 cell lysate, mouse lung tissue lysate, rat lung tissue lysate.
Conjugation
unconjugated
Clone Number
JB59-33
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000-1:5,000
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IP
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1:10-:50
Target
Function
DDAH, a dimethylarginine dimethylaminohydrolase, hydrolyzes dimethyl arginine (ADMA) and monomethyl arginine (MMA), both inhibitors of nitric oxide synthases, and may be involved in in-vivo modulation of nitric oxide production. Impairment of DDAH causes ADMA accumulation and a reduction in cGMP generation. DDAH II, the predominant DDAH isoform in endothelial cells, facilitates the induction of nitric oxide synthesis by all-trans-Retinoic acid (atRA). DDAH proteins are highly expressed in colon, kidney, stomach and liver tissues.
Background References
1. Leiper J M et al. Identification of two human dimethylarginine dimethylaminohydrolases with distinct tissue distributions and homology with microbial arginine deiminases. Biochem J 343:209-214 (1999).
2. Cillero-Pastor B et al. Dimethylarginine dimethylaminohydrolase 2, a newly identified mitochondrial protein modulating nitric oxide synthesis in normal human chondrocytes. Arthritis Rheum 64:204-212 (2012).
Sequence Similarity
Belongs to the DDAH family.
Tissue Specificity
Detected in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas, and at very low levels in brain.
Subcellular Location
Cytoplasm. Mitochondrion.
Synonyms
DDAH antibody
DDAH II antibody
DDAH-2 antibody
DDAH2 antibody
DDAH2_HUMAN antibody
DDAHII antibody
Dimethylargininase 2 antibody
Dimethylargininase-2 antibody
Dimethylarginine dimethylaminohydrolase 2 antibody
Dimethylarginine dimethylaminohydrolase II antibody
ExpandImages
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Western blot analysis of DDAH2 on different lysates with Rabbit anti-DDAH2 antibody (ET7107-41) at 1/5,000 dilution.
Lane 1: MCF7 (Human breast cancer cell) cell lysate (15 µg/Lane）
Lane 2: Mouse lung tissue lysate (30 µg/Lane）
Lane 3: Rat lung tissue lysate (30 µg/Lane）
Exposure time: 20 seconds; ECL: K1802
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET7107-41, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 30 kDa
Observed band size: 30 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"