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Proteasome 20S LMP2 Recombinant Rabbit Monoclonal Antibody [JB35-07]

Usd: 385

Specification

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Catalog# ET7107-24

Proteasome 20S LMP2 Recombinant Rabbit Monoclonal Antibody [JB35-07]

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Proteasome 20S LMP2 Recombinant Rabbit Monoclonal Antibody [JB35-07]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Proteasome 20S LMP2 aa 85-219 / 219.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P, IP

Molecular Weight

Predicted band size: 23 kDa

Positive Control

Mouse spleen tissue lysate, U937 cell lysate, mouse colon tissue lysate, human spleen tissue.

Conjugation

unconjugated

Clone Number

JB35-07

RRID

AB_3070738

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • IP

  • Use at an assay dependent concentration.

Target

Function

Proteasome subunit beta type-9 as known as 20S proteasome subunit beta-1i is a protein that in humans is encoded by the PSMB9 gene. This protein is one of the 17 essential subunits (alpha subunits 1-7, constitutive beta subunits 1-7, and inducible subunits including beta1i, beta2i, beta5i) that contributes to the complete assembly of 20S proteasome complex. In particular, proteasome subunit beta type-5, along with other beta subunits, assemble into two heptameric rings and subsequently a proteolytic chamber for substrate degradation. This protein contains "Trypsin-like" activity and is capable of cleaving after basic residues of peptide. The eukaryotic proteasome recognized degradable proteins, including damaged proteins for protein quality control purpose or key regulatory protein components for dynamic biological processes. The constitutive subunit beta1, beta2, and beta 5 (systematic nomenclature) can be replaced by their inducible counterparts beta1i, 2i, and 5i when cells are under the treatment of interferon-γ. The resulting proteasome complex becomes the so-called immunoproteasome. An essential function of the modified proteasome complex, the immunoproteasome, is the processing of numerous MHC class-I restricted T cell epitopes.

Background References

1. Akiyama K et al. Replacement of proteasome subunits X and Y by LMP7 and LMP2 induced by interferon-gamma for acquirement of the functional diversity responsible for antigen processing. FEBS Lett 343:85-88 (1994).

2. Schmidtke G et al. Analysis of mammalian 20S proteasome biogenesis: the maturation of beta-subunits is an ordered two-step mechanism involving autocatalysis. EMBO J 15:6887-6898 (1996).

Sequence Similarity

Belongs to the peptidase T1B family.

Post-translational Modification

Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.

Subcellular Location

Cytoplasm. Nucleus.

UNIPROT

SwissProt: P28065 Human

SwissProt: P28076 Mouse

Synonyms

Beta1i antibody

Large multifunctional peptidase 2 antibody

Large multifunctional protease 2 antibody

LMP 2 antibody

LMP2 antibody

Low molecular mass protein 2 antibody

Macropain chain 7 antibody

MGC70470 antibody

Multicatalytic endopeptidase complex chain 7 antibody

OTTHUMP00000062982 antibody

Expand

Images

  • Western blot analysis of Proteasome 20S LMP2 on different lysates using anti-Proteasome 20S LMP2 antibody at 1/500 dilution.<br /> <span style="font-weight: bold;">Positive control:</span><br /> Lane 1: Mouse spleen tissue lysate<br /> Lane 2: U937 cell lysate<br /> Lane 3: Mouse colon tissue lysate

    Western blot analysis of Proteasome 20S LMP2 on different lysates using anti-Proteasome 20S LMP2 antibody at 1/500 dilution.
    Positive control:
    Lane 1: Mouse spleen tissue lysate
    Lane 2: U937 cell lysate
    Lane 3: Mouse colon tissue lysate

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Proteasome 20S LMP2 antibody (<a href="/products/ET7107-24" style="font-weight: bold;text-decoration: underline;">ET7107-24</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7107-24" style="font-weight: bold;text-decoration: underline;">ET7107-24</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Proteasome 20S LMP2 antibody (ET7107-24) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-24) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation