MMP-17 Recombinant Rabbit Monoclonal Antibody [JU33-03]
Catalog# ET7106-84
MMP-17 Recombinant Rabbit Monoclonal Antibody [JU33-03]
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IF-Cell
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IF-Tissue
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IHC-P
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
MMP-17 Recombinant Rabbit Monoclonal Antibody [JU33-03]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human MMP17 aa 175-225 / 603.
Species Reactivity
Human, Mouse, Rat
Validated Applications
IF-Cell, IF-Tissue, IHC-P, WB
Target Molecular Weight
67 kDa
Positive Control
A549, MCF-7, mouse brain tissue.
Conjugation
unconjugated
Clone Number
JU33-03
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IF-Cell
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1:50
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IHC-P
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1:50
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WB
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1:500
Target
Function
Endopeptidase that degrades various components of the extracellular matrix, such as fibrin. May be involved in the activation of membrane-bound precursors of growth factors or inflammatory mediators, such as tumor necrosis factor-alpha. May also be involved in tumoral process. Cleaves pro-TNF-alpha at the '74-Ala-|-Gln-75' site. Not obvious if able to proteolytically activate progelatinase A. Does not hydrolyze collagen types I, II, III, IV and V, gelatin, fibronectin, laminin, decorin nor alpha1-antitrypsin.
Background References
1. Puente X S et al. Molecular cloning of a novel membrane-type matrix metalloproteinase from a human breast carcinoma. Cancer Res 56:944-949 (1996).
2. Martín-Alonso M et al. Deficiency of MMP17/MT4-MMP proteolytic activity predisposes to aortic aneurysm in mice. Circ Res 117:e13-26 (2015).
Sequence Similarity
Belongs to the peptidase M10A family.
Tissue Specificity
Expressed in brain, leukocytes, colon, ovary testis and breast cancer. Expressed also in many transformed and non-transformed cell types.
Post-translational Modification
The precursor is cleaved by a furin endopeptidase.
Subcellular Location
Secreted. Plasma membrane.
Synonyms
Matrix metalloproteinase 17 antibody
Matrix metalloproteinase 17 membrane inserted antibody
Matrix metalloproteinase-17 antibody
Membrane type 4 matrix metalloproteinase antibody
Membrane type matrix metalloproteinase 4 antibody
Membrane-type matrix metalloproteinase 4 antibody
Membrane-type-4 matrix metalloproteinase antibody
MMP 17 antibody
MMP-17 antibody
Mmp17 antibody
ExpandImages
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ICC staining of MMP-17 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7106-84, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of MMP-17 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7106-84, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MMP-17 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-84, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"