Coilin Recombinant Rabbit Monoclonal Antibody [JU44-08]
Catalog# ET7106-82
Coilin Recombinant Rabbit Monoclonal Antibody [JU44-08]
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WB
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Human
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Rat
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unconjugated
Overview
Product Name
Coilin Recombinant Rabbit Monoclonal Antibody [JU44-08]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Coilin aa 1-50 / 576.
Species Reactivity
Human, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 63 kDa
Positive Control
HeLa cell lysate, 293T cell lysate, PC-12 cell lysate.
Conjugation
unconjugated
Clone Number
JU44-08
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
Target
Function
Coilin protein is one of the main molecular components of Cajal bodies (CBs). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. In addition to its structural role, coilin acts as glue to connect the CB to the nucleolus. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome and chromosome.
Background References
1. Carmo-Fonseca M. al. Assembly of snRNP-containing coiled bodies is regulated in interphase and mitosis -- evidence that the coiled body is a kinetic nuclear structure. J. Cell Biol. 120:841-852(1993).
Sequence Similarity
Belongs to the coilin family.
Tissue Specificity
Found in all the cell types examined.
Post-translational Modification
Symmetrical dimethylation of arginine residues within the RG repeat region enhances affinity for SMN, and thus localization of SMN complexes to CBs.; Phosphorylation during mitosis is associated with disassembly of CBs.
Subcellular Location
Nucleus, Cajal body.
Synonyms
CLN 80 antibody
CLN80 antibody
COIL antibody
COIL_HUMAN antibody
Coilin antibody
Coilin p80 antibody
p80 antibody
P80 coilin antibody
Images
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Western blot analysis of Coilin on different lysates with Rabbit anti-Coilin antibody (ET7106-82) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 63 kDa
Observed band size: 75 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-82) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"