Skip to content

Cullin 4a Recombinant Rabbit Monoclonal Antibody [JU07-33]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# ET7106-56

Cullin 4a Recombinant Rabbit Monoclonal Antibody [JU07-33]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Green monkey

Conjugation

  • unconjugated

Overview

Product Name

Cullin 4a Recombinant Rabbit Monoclonal Antibody [JU07-33]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Cullin 4a aa 710-759 / 759.

Species Reactivity

Human, Green monkey

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 88 kDa

Positive Control

HeLa cell lysates, human breast cancer tissue, human kidney tissue, human testis tissue.

Conjugation

unconjugated

Clone Number

JU07-33

RRID

AB_3070676

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:1,000

Target

Function

Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. DCX(DDB2) ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled (H3.1) and replication-independent (H3.3) nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition.

Background References

1. Wakabayashi K. et. al. Development of a Novel Oncolytic Adenovirus Expressing a Short-hairpin RNA Against Cullin 4A. Anticancer Res. 2020 Jan

2. Sievers QL. et. al. Genome-wide screen identifies cullin-RING ligase machinery required for lenalidomide-dependent CRL4(CRBN) activity. Blood. 2018 Sep

Sequence Similarity

Belongs to the cullin family.

Post-translational Modification

Neddylated. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.; (Microbial infection) Deneddylated by Epstein-Barr virus BPLF1 leading to a S-phase-like environment that is required for efficient replication of the viral genome.

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q13619 Human

Synonyms

2810470J21Rik antibody

AW495282 antibody

CUL 4A antibody

CUL-4A antibody

Cul4a antibody

Cul4a protein antibody

CUL4A_HUMAN antibody

Cullin-4A antibody

MGC36573 antibody

MGC64071 antibody

Images

  • Western blot analysis of Cullin 4a on different lysates with Rabbit anti-Cullin 4a antibody (<a href="/products/ET7106-56" style="font-weight: bold;text-decoration: underline;">ET7106-56</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa (Human cervical adenocarcinoma cell)<br />Lane 2: SK-MEL-28 (Human malignant melanoma cell)<br />Lane 3: AGS (Human stomach adenocarcinoma cell)<br />Lane 4: HT-29 (Human colorectal adenocarcinoma cell)<br />Lane 5: COS-7 (African green monkey kidney fibroblast)<br /><br />Lysates/proteins at 10 µg/Lane.<br />Exposure time: 3 minutes; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET7106-56" style="font-weight: bold;text-decoration: underline;">ET7106-56</a>, 1/2,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 88 kDa<br />Observed band size: ~88, 92 kDa

    Western blot analysis of Cullin 4a on different lysates with Rabbit anti-Cullin 4a antibody (ET7106-56) at 1/2,000 dilution.

    Lane 1: HeLa (Human cervical adenocarcinoma cell)
    Lane 2: SK-MEL-28 (Human malignant melanoma cell)
    Lane 3: AGS (Human stomach adenocarcinoma cell)
    Lane 4: HT-29 (Human colorectal adenocarcinoma cell)
    Lane 5: COS-7 (African green monkey kidney fibroblast)

    Lysates/proteins at 10 µg/Lane.
    Exposure time: 3 minutes; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET7106-56, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 88 kDa
    Observed band size: ~88, 92 kDa

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Cullin 4a antibody (<a href="/products/ET7106-56" style="font-weight: bold;text-decoration: underline;">ET7106-56</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7106-56" style="font-weight: bold;text-decoration: underline;">ET7106-56</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Cullin 4a antibody (ET7106-56) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-56) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Cullin 4a antibody (<a href="/products/ET7106-56" style="font-weight: bold;text-decoration: underline;">ET7106-56</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7106-56" style="font-weight: bold;text-decoration: underline;">ET7106-56</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Cullin 4a antibody (ET7106-56) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-56) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Cullin 4a antibody (<a href="/products/ET7106-56" style="font-weight: bold;text-decoration: underline;">ET7106-56</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7106-56" style="font-weight: bold;text-decoration: underline;">ET7106-56</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Cullin 4a antibody (ET7106-56) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-56) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Cullin 4a antibody (<a href="/products/ET7106-56" style="font-weight: bold;text-decoration: underline;">ET7106-56</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7106-56" style="font-weight: bold;text-decoration: underline;">ET7106-56</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Cullin 4a antibody (ET7106-56) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-56) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"