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Carbonic anhydrase 2 Recombinant Rabbit Monoclonal Antibody [JU12-37]

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Specification

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Catalog# ET1706-47

Carbonic anhydrase 2 Recombinant Rabbit Monoclonal Antibody [JU12-37]

Application

  • WB

  • IHC-P

  • IHC-Fr

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Predicted reactivity

  • Cynomolgus monkey

  • Pig

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Carbonic anhydrase 2 Recombinant Rabbit Monoclonal Antibody [JU12-37]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Carbonic Anhydrase II aa 211-260 / 260.

Species Reactivity

Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

WB, IHC-P, IHC-Fr

Target Molecular Weight

Predicted band size: 29 kDa

Positive Control

Mouse kidney tissue lysate, Mouse P0 brain tissue lysate, Mouse adult brain tissue lysate, Rat kidney tissue lysate, Rat adult brain tissue lysate, 293T cell lysate, mouse brain tissue, mouse striatum tissue, rat cerebral cortex tissue, rat striatum tissue.

Conjugation

unconjugated

Clone Number

JU12-37

RRID

AB_3070667

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000-1:5,000

  • IHC-P

  • 1:200-1:1,000

  • IHC-Fr

  • 1:500

Target

Function

Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes including respiration, calcification, acid-base balance and bone resorption, as well as the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. They show extensive diversity in distribution and in their subcellular localization. The human CA2 gene, which maps to chromosome 8q21, encodes CA II, a cytoplasmic protein that has the highest turnover rate and widest tissue distribution of any known human CA isozyme. The human CA4 gene, which maps to chromosome 17q23, encodes CA IV, a membrane-anchored isozyme that is expressed on the luminal surfaces of pulmonary capillaries and proximal renal tubules. The human CA9, CA12 and CA14 genes, which map to chromosomes 9p13, 15q22 and 1q21, respectively, encode transmembrane proteins that have unique patterns of tissue-specific expression.

Background References

1. Tachibana H et al. Carbonic anhydrase 2 is a novel invasion-associated factor in urinary bladder cancers. Cancer Sci 108:331-337 (2017).

2. Gorrieri G et al. Goblet Cell Hyperplasia Requires High Bicarbonate Transport To Support Mucin Release. Sci Rep 6:36016 (2016).

Sequence Similarity

Belongs to the alpha-carbonic anhydrase family.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P00918 Human

SwissProt: P00920 Mouse

SwissProt: P27139 Rat

Synonyms

CA 2 antibody

CA II antibody

CA-II antibody

Ca2 antibody

CAC antibody

CAH2_HUMAN antibody

CAII antibody

Car 2 antibody

Car2 antibody

Carbonate dehydratase II antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Striatum <br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:500<br /><br />Antigen retrieval: Recommend. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature (recommend).

    Application: IHC-Fr

    Species: Mouse

    Site: Striatum

    Sample: Frozen section

    Antibody concentration: 1:500

    Antigen retrieval: Recommend. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature (recommend).

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebral cortex<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:500<br /><br />Antigen retrieval: Recommend. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature (recommend).

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebral cortex

    Sample: Frozen section

    Antibody concentration: 1:500

    Antigen retrieval: Recommend. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature (recommend).

  • Immunofluorescence analysis of frozen mouse cerebellum tissue with Rabbit anti-Carbonic anhydrase 2 antibody (<a href="/products/ET1706-47" style="font-weight: bold;text-decoration: underline;">ET1706-47</a>) at 1/500 dilution.<br /><br />The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature (recommend). <br /><br />The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/ET1706-47" style="font-weight: bold;text-decoration: underline;">ET1706-47</a>, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of frozen mouse cerebellum tissue with Rabbit anti-Carbonic anhydrase 2 antibody (ET1706-47) at 1/500 dilution.

    The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature (recommend).

    The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1706-47, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • Western blot analysis of Carbonic anhydrase 2 on different lysates with Rabbit anti-Carbonic anhydrase 2 antibody (<a href="/products/ET1706-47" style="font-weight: bold;text-decoration: underline;">ET1706-47</a>) at 1/5,000 dilution.<br /><br />Lane 1: HEK-293 (Human embryonic kidney cell)<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 10 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET1706-47" style="font-weight: bold;text-decoration: underline;">ET1706-47</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 29 kDa<br />Observed band size: 29 kDa

    Western blot analysis of Carbonic anhydrase 2 on different lysates with Rabbit anti-Carbonic anhydrase 2 antibody (ET1706-47) at 1/5,000 dilution.

    Lane 1: HEK-293 (Human embryonic kidney cell)

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 10 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET1706-47, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 29 kDa
    Observed band size: 29 kDa

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Carbonic anhydrase 2 on different lysates with Rabbit anti-Carbonic anhydrase 2 antibody (<a href="/products/ET1706-47" style="font-weight: bold;text-decoration: underline;">ET1706-47</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse kidney tissue lysate<br />Lane 2: Mouse P0 brain tissue lysate<br />Lane 3: Mouse adult brain tissue lysate<br />Lane 4: Rat kidney tissue lysate<br />Lane 5: Rat adult brain tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 29 kDa<br />Observed band size: 29 kDa<br /><br />Exposure time: 6 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1706-47" style="font-weight: bold;text-decoration: underline;">ET1706-47</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Carbonic anhydrase 2 on different lysates with Rabbit anti-Carbonic anhydrase 2 antibody (ET1706-47) at 1/2,000 dilution.

    Lane 1: Mouse kidney tissue lysate
    Lane 2: Mouse P0 brain tissue lysate
    Lane 3: Mouse adult brain tissue lysate
    Lane 4: Rat kidney tissue lysate
    Lane 5: Rat adult brain tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 29 kDa
    Observed band size: 29 kDa

    Exposure time: 6 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-47) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Carbonic anhydrase 2 antibody (<a href="/products/ET1706-47" style="font-weight: bold;text-decoration: underline;">ET1706-47</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1706-47" style="font-weight: bold;text-decoration: underline;">ET1706-47</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Carbonic anhydrase 2 antibody (ET1706-47) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-47) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rabbit anti-Carbonic anhydrase 2 antibody (<a href="/products/ET1706-47" style="font-weight: bold;text-decoration: underline;">ET1706-47</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1706-47" style="font-weight: bold;text-decoration: underline;">ET1706-47</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rabbit anti-Carbonic anhydrase 2 antibody (ET1706-47) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-47) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue with Rabbit anti-Carbonic anhydrase 2 antibody (<a href="/products/ET1706-47" style="font-weight: bold;text-decoration: underline;">ET1706-47</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1706-47" style="font-weight: bold;text-decoration: underline;">ET1706-47</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue with Rabbit anti-Carbonic anhydrase 2 antibody (ET1706-47) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-47) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat striatum tissue with Rabbit anti-Carbonic anhydrase 2 antibody (<a href="/products/ET1706-47" style="font-weight: bold;text-decoration: underline;">ET1706-47</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1706-47" style="font-weight: bold;text-decoration: underline;">ET1706-47</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat striatum tissue with Rabbit anti-Carbonic anhydrase 2 antibody (ET1706-47) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-47) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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