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HSD11B1 Recombinant Rabbit Monoclonal Antibody [JU18-39]

Usd: 385

Specification

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Catalog# ET1706-27

HSD11B1 Recombinant Rabbit Monoclonal Antibody [JU18-39]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

HSD11B1 Recombinant Rabbit Monoclonal Antibody [JU18-39]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human HSD11B1 aa 1-50 / 292.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 32 kDa

Positive Control

Mouse liver tissue lysate, Rat liver tissue lysate, human liver tissue, mouse liver tissue.

Conjugation

unconjugated

Clone Number

JU18-39

RRID

AB_3070650

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:50-1:200

Target

Function

HSD11B1(Corticosteroid 11-beta-dehydrogenase isozyme 1) is also named as HSD11, HSD11L,11-DH and 11-beta-HSD1.It is a microsomal enzyme, which acts mainly as a NADP(H)- dependent reductase converting inactive cortisone (E) to active cortisol (F), thus regulating intracellular F access to glucocorticoid receptor. This enzyme is widely expressed not only in liver and adipose tissue, but also in adrenal, ovary and decidua.Western blot analysis in human liver, using a specific11-beta-HSD1 antibody, showed two bands at approximately 34 and 68 kDa confrming the dimeric nature of 11-beta-HSD1 in liver and In omental adipose tissue, an additional band of 50 kDa was strongly expressed.

Background References

1. Zeng X et al. Lysine-specific demethylase 1 promotes brown adipose tissue thermogenesis via repressing glucocorticoid activation. Genes Dev 30:1822-36 (2016).

2. McGee-Lawrence ME et al. Hdac3 Deficiency Increases Marrow Adiposity and Induces Lipid Storage and Glucocorticoid Metabolism in Osteochondroprogenitor Cells. J Bone Miner Res 31:116-28 (2016).

Sequence Similarity

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Tissue Specificity

Widely expressed. Highest expression in liver.

Post-translational Modification

Glycosylated.

Subcellular Location

Endoplasmic reticulum, Membrane.

UNIPROT

SwissProt: P28845 Human

SwissProt: P50172 Mouse

SwissProt: P16232 Rat

Synonyms

11 beta HSD 1 antibody

11 beta HSD1 antibody

11 beta hydroxysteroid dehydrogenase 1 antibody

11 DH antibody

11-beta hydroxysteroid dehydrogenase, type 1 antibody

11-beta-HSD1 antibody

11-beta-hydroxysteroid dehydrogenase 1 antibody

11-DH antibody

11DH antibody

Corticosteroid 11 beta dehydrogenase isozyme 1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of HSD11B1 on different lysates with Rabbit anti-HSD11B1 antibody (<a href="/products/ET1706-27" style="font-weight: bold;text-decoration: underline;">ET1706-27</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse liver tissue lysate<br />Lane 2: Mouse brain tissue lysate (negative)<br />Lane 3: Rat liver tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 32 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1706-27" style="font-weight: bold;text-decoration: underline;">ET1706-27</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of HSD11B1 on different lysates with Rabbit anti-HSD11B1 antibody (ET1706-27) at 1/2,000 dilution.

    Lane 1: Mouse liver tissue lysate
    Lane 2: Mouse brain tissue lysate (negative)
    Lane 3: Rat liver tissue lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 32 kDa
    Observed band size: 35 kDa

    Exposure time: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-27) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-HSD11B1 antibody (<a href="/products/ET1706-27" style="font-weight: bold;text-decoration: underline;">ET1706-27</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1706-27" style="font-weight: bold;text-decoration: underline;">ET1706-27</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-HSD11B1 antibody (ET1706-27) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-27) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-HSD11B1 antibody (<a href="/products/ET1706-27" style="font-weight: bold;text-decoration: underline;">ET1706-27</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1706-27" style="font-weight: bold;text-decoration: underline;">ET1706-27</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-HSD11B1 antibody (ET1706-27) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-27) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"