NUP153 Recombinant Rabbit Monoclonal Antibody [JU40-41]
Catalog# ET1706-25
NUP153 Recombinant Rabbit Monoclonal Antibody [JU40-41]
-
WB
-
Human
-
unconjugated
Overview
Product Name
NUP153 Recombinant Rabbit Monoclonal Antibody [JU40-41]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human NUP153 aa 51-100 / 1,475.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 154 kDa
Positive Control
HeLa cell lysate, K-562 cell lysate.
Conjugation
unconjugated
Clone Number
JU40-41
RRID
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | IF-Cell | |
|---|---|---|---|
| human |
|
|
|
| mouse |
|
|
|
| rat |
|
|
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500-1:2,000
Target
Function
Nuclear pore complexes (NPCs) are the channels for the bi-directional movement of macromolecules between the nucleus and cytoplasm, and contain more than 100 different subunits. Many of them belong to a family called nucleoporins, which are characterized by the presence of O-linked N-acetylglucosamine moieties and a distinctive pentapeptide repeat (XFXFG). Nup153 is a peripheral NPC component that is implicated in protein and RNP transport and in the interaction of NPCs with the nuclear lamina. Nup153 contains a unique N-terminal region, a central domain consisting of four to five zinc fingers and a C-terminal region containing about 30 irregularly spaced FXFG repeats. Nup153 is cleaved by caspases during apoptosis. Nup153 interacts wtih TAP, which is essential for mRNA export, and associates with chromatin towards the end of anaphase, in parallel with the inner nuclear membrane protein LAP2. Nup153 is involved in NPC assembly, in anchoring NPCs within the nuclear envelope and in mediating specific nuclear import events.
Background References
1. Mamede JI et al. Cyclophilins and nucleoporins are required for infection mediated by capsids from circulating HIV-2 primary isolates. Sci Rep 7:45214 (2017).
2. Winter FR et al. Multicolour nanoscopy of fixed and living cells with a single STED beam and hyperspectral detection. Sci Rep 7:46492 (2017).
Sequence Similarity
Belongs to the NUP153 family.
Post-translational Modification
Phosphorylated in interphase, hyperphosphorylated during mitosis. May play a role in the reversible disassembly of the nuclear pore complex during mitosis (By similarity).; Proteolytically degraded after poliovirus (PV) infection; degradation is partial and NCP- and TPR-binding domains withstand degradation.; O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
Subcellular Location
Nucleus, nuclear pore complex.
UNIPROT
Synonyms
153 kDa nucleoporin antibody
HNUP153 antibody
N153 antibody
NU153_HUMAN antibody
Nuclear pore complex protein hnup153 antibody
Nuclear pore complex protein Nup153 antibody
Nucleoporin 153kDa antibody
Nucleoporin Nup153 antibody
Nup 153 antibody
Nup153 antibody
Images
-
Western blot analysis of NUP153 on different lysates with Rabbit anti-NUP153 antibody (ET1706-25) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 154 kDa
Observed band size: 154 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-25) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"