HP1 gamma Recombinant Rabbit Monoclonal Antibody [JU81-36]
Catalog# ET1706-02
HP1 gamma Recombinant Rabbit Monoclonal Antibody [JU81-36]
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WB
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IHC-P
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FC
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IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
HP1 gamma Recombinant Rabbit Monoclonal Antibody [JU81-36]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human HP1 gamma aa 38-87 / 183.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, FC, IP
Target Molecular Weight
Predicted band size: 21 kDa
Positive Control
HeLa, MCF7,NIH/3T3,C6,PC-12,mouse colon tissue lysates, rat testis tissue, human liver carcinoma tissue, human tonsil tissue, human placenta tissue, mouse colon tissue, mouse testis tissue, Hela.
Conjugation
unconjugated
Clone Number
JU81-36
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
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IP
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1:10-1:50
Target
Function
Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of Histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as Histone H3 is dephosphorylated.
Background References
1. Lastwika KJ et al. Control of PD-L1 Expression by Oncogenic Activation of the AKT-mTOR Pathway in Non-Small Cell Lung Cancer. Cancer Res 76:227-38 (2016).
2. Rielland M et al. Senescence-associated SIN3B promotes inflammation and pancreatic cancer progression. J Clin Invest 124:2125-35 (2014).
Post-translational Modification
Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
Subcellular Location
Nucleus.
Synonyms
CBX 3 antibody
CBX3 antibody
CBX3_HUMAN antibody
Chromobox homolog 3 (HP1 gamma homolog, Drosophila) antibody
Chromobox homolog 3 antibody
Chromobox protein homolog 3 antibody
GAMMA antibody
HECH antibody
Heterochromatin like protein 1 antibody
Heterochromatin protein 1 homolog gamma antibody
ExpandImages
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Western blot analysis of HP1 gamma on different lysates with Rabbit anti-HP1 gamma antibody (ET1706-02) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: MCF7 (Human breast cancer cell) cell lysate
Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate
Lane 4: C6 (Rat glioma cell) cell lysate
Lane 5: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate
Cell Lysates at 15 µg/Lane
Exposure time: 60 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET1706-02, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 21 kDa
Observed band size: 21 kDa -
Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-HP1 gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-02, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-HP1 gamma antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-02, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HP1 gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-02, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-HP1 gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-02, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-HP1 gamma antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-02, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-HP1 gamma antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-02, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of HP1 gamma was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1706-02, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"