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Dynein intermediate chain 1 Recombinant Rabbit Monoclonal Antibody [JM11-38]

Usd: 385

Specification

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Catalog# ET1705-8

Dynein intermediate chain 1 Recombinant Rabbit Monoclonal Antibody [JM11-38]

Application

  • WB

  • IHC-P

Reactivity

  • Mouse

Predicted reactivity

  • Human

  • Rat

Predicted species support after-sales service
Conjugation

  • unconjugated

Overview

Product Name

Dynein intermediate chain 1 Recombinant Rabbit Monoclonal Antibody [JM11-38]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Dynein intermediate chain 1 aa 1-240 / 699.

Species Reactivity

Mouse (Predicted: Human, Rat)

Predicted species support after-sales service

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 79 kDa

Positive Control

Mouse fallopian tube tissue, mouse testis tissue.

Conjugation

unconjugated

Clone Number

JM11-38

RRID

AB_3070608

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

Target

Function

Dyneins are multisubunit, high molecular weight ATPases that interact with microtubules to generate force by converting the chemical energy of ATP into the mechanical energy of movement. Cytoplasmic or axonemal Dynein heavy, intermediate, light and light-intermediate chains are all components of minus end-directed motors; the complex transports cellular cargos towards the central region of the cell. Axonemal Dynein motors contain one to three non-identical heavy chains and cause a sliding of microtubules in the axonemes of cilia and flagella in a mechanism necessary for cilia to beat and propel the cell. Cytoplasmic Dyneins, such as Dynein IC1, cytosolic and Dynein IC2, cytosolic, comprise an approximately 12 subunit complex of two heavy chains, two intermediate chains to anchor Dynein to its cargo, four smaller intermediate chains and several light chains. This complex performs functions necessary for cell survival, such as organelle transport and centrosome assembly. The carboxy terminus of Dynein is important for microtubule-dependent motility and is highly conserved, while the amino terminal regions are more variable. Several proteins regulate Dynein activity, including dynactin, LIS1 and NudEL(NudE-like).

Background References

1. Mahale SP et al. Dynein Light Intermediate Chain 2 Facilitates the Metaphase to Anaphase Transition by Inactivating the Spindle Assembly Checkpoint. PLoS One 11:e0159646 (2016).

2. Pillai S et al. Tank binding kinase 1 is a centrosome-associated kinase necessary for microtubule dynamics and mitosis. Nat Commun 6:10072 (2015).

Sequence Similarity

Belongs to the dynein intermediate chain family.

Subcellular Location

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Dynein, Microtubule.

UNIPROT

SwissProt: Q9UI46 Human

SwissProt: Q8C0M8 Mouse

SwissProt: Q5XIL8 Rat

Synonyms

Axonemal dynein intermediate chain 1 antibody

Axonemal dynein intermediate chain 2 antibody

CILD 1 antibody

CILD1 antibody

Cytoplasmic dynein 1 intermediate chain 1 antibody

Cytoplasmic dynein 1 intermediate chain 2 antibody

Cytoplasmic dynein intermediate chain 1 antibody

Cytoplasmic dynein intermediate chain 2 antibody

DH IC 1 antibody

DH IC 2 antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-Dynein intermediate chain 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1705-8" style="font-weight: bold;text-decoration: underline;">ET1705-8</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-Dynein intermediate chain 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-8, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Dynein intermediate chain 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1705-8" style="font-weight: bold;text-decoration: underline;">ET1705-8</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Dynein intermediate chain 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-8, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"