DDAH1 Recombinant Rabbit Monoclonal Antibody [JM53-18]
Catalog# ET1705-71
DDAH1 Recombinant Rabbit Monoclonal Antibody [JM53-18]
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WB
-
IP
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Human
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unconjugated
Overview
Product Name
DDAH1 Recombinant Rabbit Monoclonal Antibody [JM53-18]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human DDAH1 aa 100-285 / 285.
Species Reactivity
Human
Validated Applications
WB, IP
Target Molecular Weight
Predicted band size: 31 kDa
Positive Control
SH-SY5Y cell lysate, HepG2 cell lysate, HUVEC cell lysate, human prostate tissue, human kidney tissue.
Conjugation
unconjugated
Clone Number
JM53-18
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IP
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Use at an assay dependent concentration.
Target
Function
DDAH, a dimethylarginine dimethylaminohydrolase, hydrolyzes dimethyl arginine (ADMA) and monomethyl arginine (MMA), both inhibitors of nitric oxide synthases, and may be involved in in-vivo modulation of nitric oxide production. Impairment of DDAH causes ADMA accumulation and a reduction in cGMP generation. DDAH II, the predominant DDAH isoform in endothelial cells, facilitates the induction of nitric oxide synthesis by all-trans-Retinoic acid (atRA). DDAH proteins are highly expressed in colon, kidney, stomach and liver tissues.
Background References
1. Wang H et al. DDAH1 plays dual roles in PM2.5 induced cell death in A549 cells. Biochim Biophys Acta 1860:2793-801 (2016).
2. Jung CS et al. Endogenous nitric-oxide synthase inhibitor ADMA after acute brain injury. Int J Mol Sci 15:4088-103 (2014).
Sequence Similarity
Belongs to the DDAH family.
Tissue Specificity
Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle.
Subcellular Location
Cytoplasm.
UNIPROT
Synonyms
DDAH antibody
DDAH I antibody
DDAH-1 antibody
DDAH1 antibody
DDAH1_HUMAN antibody
DDAHI antibody
Dimethylargininase 1 antibody
Dimethylargininase-1 antibody
Dimethylarginine dimethylaminohydrolase 1 antibody
N(G) antibody
ExpandImages
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☑ Knockdown (KD)
Western blot analysis of DDAH1 on different lysates with Rabbit anti-DDAH1 antibody (ET1705-71) at 1/5,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-DDAH1 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 37 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-71) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of DDAH1 on different lysates with Rabbit anti-DDAH1 antibody (ET1705-71) at 1/5,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: HepG2 cell lysate
Lane 3: HUVEC cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 37 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-71) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"