CD1a Recombinant Rabbit Monoclonal Antibody [JM21-33]
Catalog# ET1705-17
CD1a Recombinant Rabbit Monoclonal Antibody [JM21-33]
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WB
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IHC-P
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IF-Tissue
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Human
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unconjugated
Overview
Product Name
CD1a Recombinant Rabbit Monoclonal Antibody [JM21-33]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human CD1a aa 16-65 / 327.
Species Reactivity
Human
Validated Applications
WB, IHC-P, IF-Tissue
Target Molecular Weight
Predicted band size: 37 kDa
Positive Control
Jurkat cell lysate, MOLT-4 cell lysate, human thymoma tissue, human thymus tissue, human skin tissue, human tonsil tissue.
Conjugation
unconjugated
Clone Number
JM21-33
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IHC-P
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1:200-1:500
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IF-Tissue
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1:2,000
Target
Function
CD1a (Cluster of Differentiation 1a) is a human protein encoded by the CD1A gene. This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to the plasma membrane and to recycling vesicles of the early endocytic system. Alternatively spliced transcript variants have been observed, but their biological validity has not been determined. Transcript levels of the CD1A gene are upregulated in the lung parenchyma of smokers.
Background References
1. Nguyen N et al. Tumor infiltrating lymphocytes and survival in patients with head and neck squamous cell carcinoma. Head Neck 38:1074-84 (2016).
2. Tam J et al. Reconstitution of full-thickness skin by microcolumn grafting. J Tissue Eng Regen Med doi: 10.1002/term.2174 (2016).
Tissue Specificity
Expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues.
Subcellular Location
Cell membrane. Endosome membrane.
UNIPROT
Synonyms
CD 1a antibody
CD1 antibody
CD1a antibody
CD1A Antigen antibody
CD1A antigen, a polypeptide antibody
CD1a molecule antibody
CD1A_HUMAN antibody
cluster of differentiation 1 A antibody
cortical thymocyte antigen CD1A antibody
differentiation antigen CD1 alpha 3 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of CD1a on different lysates with Rabbit anti-CD1a antibody (ET1705-17) at 1/2,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: MOLT-4 cell lysate
Lane 3: HeLa cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 37 kDa
Observed band size: 50 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-17) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Application: IF-Tissue
Species: Human
Site: skin
Sample: Paraffin-embedded section
Antibody concentration: 1/2,000 -
Immunohistochemical analysis of paraffin-embedded human thymoma tissue with Rabbit anti-CD1a antibody (ET1705-17) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-17) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human thymus tissue with Rabbit anti-CD1a antibody (ET1705-17) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-17) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-CD1a antibody (ET1705-17) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-17) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD1a antibody (ET1705-17) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-17) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded human brain tissue (negative) with Rabbit anti-CD1a antibody (ET1705-17) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-17) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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