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Periostin Recombinant Rabbit Monoclonal Antibody [JA63-40]

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Specification

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Catalog# ET1704-65

Periostin Recombinant Rabbit Monoclonal Antibody [JA63-40]

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Periostin Recombinant Rabbit Monoclonal Antibody [JA63-40]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic pepride within Human Perilipin-1 aa 11-60 / 836.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 93 kDa

Positive Control

HaCaT cell lysate, HFF-1 cell lysate, BxPC-3 cell lysate, SH-SY5Y cell lysate, 293T cell lysate, human skin tissue lysate, human lung tissue lysate, human brain tissue lysate.

Conjugation

unconjugated

Clone Number

JA63-40

RRID

AB_3070511

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Periostin (PN), also designated osteoblast-specific factor 2 (OSF-2), is a disulfide linked protein originally isolated as a osteoblast-specific factor. Periostin is a secreted protein that binds heparin and functions as a ligand for alpha(V)beta and alpha(V)beta integrins. In preosteoblasts, Periostin acts as a cell adhesion molecule and plays a role in osteoblast recruitment, spreading and attachment. Periostin is mainly detected in lower gastrointestinal tract, aorta, stomach, placenta, uterus and breast tissues but is up-regulated in epithelial ovarian tumors and overexpressed in breast cancer. Expression of Periostin is increased by bone morphogenetic protein (BMP2) and transforming growth factor β 1(TGF β 1). Periostin contains a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain, which shows homology with the insect protein fasciclin, and a C-terminal domain.

Background References

1. Ma H. et. al. Periostin Promotes Colorectal Tumorigenesis through Integrin-FAK-Src Pathway-Mediated YAP/TAZ Activation. Cell Rep. 2020 Jan

2. Kormann R. et. al. Periostin Promotes Cell Proliferation and Macrophage Polarization to Drive Repair after AKI. J Am Soc Nephrol. 2020 Jan

Tissue Specificity

Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus, thyroid tissue and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers.

Post-translational Modification

Gamma-carboxylation is controversial. Gamma-carboxyglutamated; gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation; this may be required for calcium binding. According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues.

Subcellular Location

Golgi apparatus, Secreted, extracellular space, extracellular matrix.

UNIPROT

SwissProt: Q15063 Human

Synonyms

Fasciclin-I like antibody

MGC119510 antibody

MGC119511 antibody

OSF 2 antibody

OSF-2 antibody

OSF2 antibody

Osteoblast specific factor 2 (fasciclin I like) antibody

Osteoblast specific factor 2 antibody

Osteoblast specific factor antibody

Osteoblast-specific factor 2 antibody

Expand

Images

  • Western blot analysis of Periostin on different lysates with Rabbit anti-Periostin antibody (<a href="/products/ET1704-65" style="font-weight: bold;text-decoration: underline;">ET1704-65</a>) at 1/1,000 dilution.<br /><br />Lane 1: HaCaT cell lysate<br />Lane 2: HFF-1 cell lysate<br />Lane 3: BxPC-3 cell lysate<br />Lane 4: SH-SY5Y cell lysate<br />Lane 5: 293T cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 93 kDa<br />Observed band size: 85 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1704-65" style="font-weight: bold;text-decoration: underline;">ET1704-65</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Periostin on different lysates with Rabbit anti-Periostin antibody (ET1704-65) at 1/1,000 dilution.

    Lane 1: HaCaT cell lysate
    Lane 2: HFF-1 cell lysate
    Lane 3: BxPC-3 cell lysate
    Lane 4: SH-SY5Y cell lysate
    Lane 5: 293T cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 93 kDa
    Observed band size: 85 kDa

    Exposure time: 20 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-65) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Periostin on different lysates with Rabbit anti-Periostin antibody (<a href="/products/ET1704-65" style="font-weight: bold;text-decoration: underline;">ET1704-65</a>) at 1/1,000 dilution.<br /><br />Lane 1: Human skin tissue lysate<br />Lane 2: Human lung tissue lysate<br />Lane 3: Human brain tissue lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 93 kDa<br />Observed band size: 85 kDa<br /><br />Exposure time: Lane 1: 3 minutes; Lane 2-3: 24 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1704-65" style="font-weight: bold;text-decoration: underline;">ET1704-65</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Periostin on different lysates with Rabbit anti-Periostin antibody (ET1704-65) at 1/1,000 dilution.

    Lane 1: Human skin tissue lysate
    Lane 2: Human lung tissue lysate
    Lane 3: Human brain tissue lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 93 kDa
    Observed band size: 85 kDa

    Exposure time: Lane 1: 3 minutes; Lane 2-3: 24 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-65) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation