TLR7 Recombinant Rabbit Monoclonal Antibody [JA93-11]
Catalog# ET1704-11
TLR7 Recombinant Rabbit Monoclonal Antibody [JA93-11]
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WB
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Human
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unconjugated
Overview
Product Name
TLR7 Recombinant Rabbit Monoclonal Antibody [JA93-11]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human TLR7 aa 1000-1049 / 1049.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 121 kDa
Positive Control
Jurkat cell lysate, Daudi cell lysate.
Conjugation
unconjugated
Clone Number
JA93-11
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
Target
Function
The Toll-like Receptors (TLR) are a family of human receptors that share homology with the Drosophila Toll Receptors, which are involved in mediating dorsoventral polarization in developing Drosophila embryos and participate in host immunity. The TLR family members are characterized by a highly conserved Toll homology (TH) domain, which is essential for Toll-induced signal transductions. TLRs are type I transmembrane receptors that contain an extracellular domain consisting of several leucine-rich regions and a single cytoplasmic Toll/IL-1R like domain. Three TLR family members, TLR7, TLR8 and TLR9, belong to a subfamily of TLRs which are differentially expressed. TLR7 is expressed in lung, placenta and spleen. TLR8 is expressed in lung and peripheral blood leukocytes, and TLR9 is predominantly expressed in spleen, lymph nodes, bone marrow and peripheral blood leukocytes. TLR7, TLR8 and TLR9 stimulate the NFκB signaling pathway, suggesting that they play a role in the immune response.
Background References
1. Tao Y et al. The role of endogenous IFN- in the regulation of Th17 responses in patients with relapsing-remitting multiple sclerosis. J Immunol 192:5610-7 (2014).
2. Chen GY et al. Simultaneous induction of autophagy and toll-like receptor signaling pathways by graphene oxide. Biomaterials 33:6559-69 (2012).
Sequence Similarity
Belongs to the Toll-like receptor family.
Tissue Specificity
Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells.
Subcellular Location
Endoplasmic reticulum membrane, Endosome, Lysosome, Cytoplasmic vesicle, phagosome.
UNIPROT
Synonyms
PRO285 antibody
TLR 7 antibody
Tlr7 antibody
TLR7_HUMAN antibody
Toll like receptor 7 antibody
Toll-like receptor 7 antibody
UNQ248 antibody
Images
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Western blot analysis of TLR7 on different lysates with Rabbit anti-TLR7 antibody (ET1704-11) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: Jurkat cell lysate (hot lysis)
Lane 3: Daudi cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 121 kDa
Observed band size: 140/70 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-11) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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