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Calcineurin A Recombinant Rabbit Monoclonal Antibody [JA64-11]

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Specification

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Catalog# ET1704-02

Calcineurin A Recombinant Rabbit Monoclonal Antibody [JA64-11]

Application

  • WB

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Calcineurin A Recombinant Rabbit Monoclonal Antibody [JA64-11]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Calcineurin A aa 472-521 / 521.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, FC

Molecular Weight

Predicted band size: 59 kDa

Positive Control

MCF7 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, Hela cell lysate, 293 cell lysate, A431 cell lysate, mouse cerebellum tissue lysate, Hela.

Conjugation

unconjugated

Clone Number

JA64-11

RRID

AB_3070455

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • FC

  • 1:50-1:100

Target

Function

In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunit have been identified, designated PP1, PP2A, PP2B and PP2C. An additional protein phosphatase catalytic subunit, PPX (also known as PP4), is a putative member of a novel PP family. The PP2B family comprises subfamily members PP2B-Aα, PP2B-Aβ and PP2B-Aγ. Two additional regulatory subunits been identified, designated PP2B-B1 and PP2B-B2.

Background References

1. Turdi S et al. Interaction between maternal and postnatal high fat diet leads to a greater risk of myocardial dysfunction in offspring via enhanced lipotoxicity, IRS-1 serine phosphorylation and mitochondrial defects. J Mol Cell Cardiol 55:117-29 (2013).

2. Thoms KM et al. Cyclosporin A, but not everolimus, inhibits DNA repair mediated by calcineurin: implications for tumorigenesis under immunosuppression. Exp Dermatol 20:232-6 (2011).

Sequence Similarity

Belongs to the PPP phosphatase family. PP-2B subfamily.

Tissue Specificity

Expressed in keratinocytes (at protein level).

Subcellular Location

Cell membrane, Cytoplasm.

UNIPROT

SwissProt: Q08209 Human

SwissProt: P63328 Mouse

SwissProt: P63329 Rat

Synonyms

Alpha isoform formerly PPP2B antibody

Calcineurin A alpha antibody

Calcineurin A1 antibody

CalcineurinA antibody

Calmodulin dependent calcineurin A subunit alpha isoform antibody

Calmodulin-dependent calcineurin A subunit alpha isoform antibody

CALN antibody

CALNA 1 antibody

CALNA antibody

CALNA1 antibody

Expand

Images

  • Western blot analysis of Calcineurin A on different lysates with Rabbit anti-Calcineurin A antibody (<a href="/products/ET1704-02" style="font-weight: bold;text-decoration: underline;">ET1704-02</a>) at 1/1,000 dilution.<br /><br />Lane 1: MCF7 cell lysate (20 µg/Lane)<br />Lane 2: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 3: Rat brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 59 kDa<br />Observed band size: 59 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1704-02" style="font-weight: bold;text-decoration: underline;">ET1704-02</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Calcineurin A on different lysates with Rabbit anti-Calcineurin A antibody (ET1704-02) at 1/1,000 dilution.

    Lane 1: MCF7 cell lysate (20 µg/Lane)
    Lane 2: Mouse brain tissue lysate (40 µg/Lane)
    Lane 3: Rat brain tissue lysate (40 µg/Lane)

    Predicted band size: 59 kDa
    Observed band size: 59 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-02) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Calcineurin A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET1704-02" style="font-weight: bold;text-decoration: underline;">ET1704-02</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.<br /><span style="font-weight: bold;">Positive control:</span> <br />Lane 1: Hela cell lysate<br />Lane 2: 293 cell lysate<br />Lane 3: A431 cell lysate

    Western blot analysis of Calcineurin A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-02, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Hela cell lysate
    Lane 2: 293 cell lysate
    Lane 3: A431 cell lysate

  • Western blot analysis of Calcineurin A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET1704-02" style="font-weight: bold;text-decoration: underline;">ET1704-02</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.<br /><span style="font-weight: bold;">Positive control:</span> <br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Mouse cerebellum tissue lysate<br />Lane 3: Rat brain tissue lysate

    Western blot analysis of Calcineurin A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-02, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Mouse brain tissue lysate
    Lane 2: Mouse cerebellum tissue lysate
    Lane 3: Rat brain tissue lysate

  • Flow cytometric analysis of Calcineurin A was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ET1704-02" style="font-weight: bold;text-decoration: underline;">ET1704-02</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Calcineurin A was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-02, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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Citation